Thermo Fisher Scientific eIF3h Polyclonal Antibody
Rabbit polyclonal antibody against human eIF3h, validated for WB, IHC, ICC/IF, and IP. Affinity-purified with >95% purity. Supports research on translation initiation and cell proliferation pathways. For research use only.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| Immunoprecipitation (IP) | 1:50–1:100 |
Product Specifications
| Specification | Description |
|---|---|
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant full-length Human EIF3H (amino acids 1–352; UniProt ID: O15372-1) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Storage Conditions | Store at 4 °C for short term. For long-term storage, store at −20 °C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2720829 |
Product Specific Information
The antibody was affinity-purified from rabbit antiserum using an epitope-specific immunogen. Purity is greater than 95% as determined by SDS-PAGE.
Target Information
eIF3H is a component of the eukaryotic translation initiation factor 3 (eIF-3) complex, essential for multiple steps in protein synthesis initiation. The eIF-3 complex associates with the 40S ribosomal subunit and facilitates recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi, and eIF-5 to form the 43S pre-initiation complex (43S PIC). It promotes mRNA recruitment, scanning for AUG recognition, and participates in ribosomal recycling and prevention of premature subunit joining. eIF3H selectively regulates translation of mRNAs involved in cell proliferation, differentiation, and apoptosis through RNA stem-loop binding mechanisms.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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