Thermo Fisher Scientific CD45 Monoclonal Antibody (2D1), NovaFluor Red 725, eBioscience

Applications

• Flow Cytometry (Flow)
  Tested dilution: 5 µL (0.8 µg)/test

Product Specifications

Additional Available Formats

• Alexa Fluor 700
• APC
• APC-eFluor 780
• Brilliant Violet 711
• eFluor 450
• FITC
• NovaFluor Blue Series (510~800)
• NovaFluor Red Series (660~755)
• NovaFluor UV/Violet/Yellow Series
• PE, PE-Cyanine7, PE-eFluor 610
• PerCP-Cyanine5.5
• Super Bright Series (436~780)
• Custom conjugation available upon request

Product Specific Information

The 2D1 monoclonal antibody reacts with all isoforms of human CD45 (Leukocyte Common Antigen, LCA). CD45 is expressed on all hematopoietic cells except circulating erythrocytes and platelets. The cytoplasmic domain has tyrosine phosphatase activity, crucial for lymphocyte activation.

Each product includes:

• 1 vial of NovaFluor conjugate
• 1 vial of CellBlox Plus Blocking Buffer

Applications Reported: Flow cytometric analysis
Applications Tested: Pre-diluted and tested for flow cytometry using human peripheral blood cells. Recommended use: 5 µL (0.8 µg) per test (100 µL final volume, 10⁵–10⁸ cells/test).

Important Notes:

• NovaFluor dyes are not compatible with DNA intercalating viability dyes (e.g., propidium iodide, 7-AAD, DAPI).
• Use Invitrogen LIVE/DEAD Fixable Dead Cell stains for compatibility.
• CellBlox Plus Blocking Buffer (Cat. No. C001T06F01) is required for optimal staining.
  • Add 5 µL per sample to antibody cocktail.
  • For single-color controls: 5 µL per 100 µL cell sample (10³–10⁸ cells).
• NovaFluor conjugates utilize Phiton™ technology, providing engineered fluorescent signatures optimized for reduced spillover and spread.

Excitation: 636 nm
Emission: 727 nm
Laser: 633–640 nm (Red)

Target Information

CD45 (LCA) is a receptor-type protein tyrosine phosphatase expressed on all nucleated hematopoietic cells (~10% of lymphocyte surface proteins). It is absent in non-hematopoietic tissues. CD45 regulates lymphocyte development, antigen receptor signaling, and cytokine signaling via modulation of Src-family kinases and JAK kinases. Isoforms arise from alternative splicing and differ in extracellular domains. CD45 also influences integrin-mediated signaling, apoptosis, and immune regulation.

For Research Use Only. Not for diagnostic procedures or resale without authorization.

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