Thermo Fisher Scientific Acrolein Monoclonal Antibody (10A10), PE
Acrolein 변형 단백질을 특이적으로 인식하는 PE 결합 단클론 항체로, WB, IHC, ICC/IF, Flow Cytometry, ELISA에 사용 가능. Mouse IgG1 아이소타입이며, 단백질 G로 정제된 액상 형태. 형광 특성은 Ex/Em 565/576 nm.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (IHC) | 1:100 |
| Immunocytochemistry (ICC/IF) | 1:50 |
| Flow Cytometry (Flow) | 1:100 |
| ELISA | 1:1,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Chemical |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 10A10 |
| Immunogen | Synthetic Acrolein modified Keyhole Limpet Hemocyanin (KLH) |
| Conjugate | PE (R-Phycoerythrin) |
| Excitation / Emission Max | 565 / 576 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | 95.64 mM phosphate / 2.48 mM MES, pH 7.4, with 0.5 M EDTA |
| Contains | No preservative |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2932222 |
Available Formats
- Unconjugated (Cat. MA5-27553)
- APC (Cat. MA5-45765)
- FITC (Cat. MA5-45766)
- PerCP (Cat. MA5-45767)
- Custom conjugation available upon request
Product Specific Information
A 1:1,000 dilution of MA5-45768 was sufficient for detection of Acrolein in 2 µg of Acrolein conjugated to BSA by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary antibody.
Specific for Acrolein-modified proteins. Does not detect free Acrolein. Does not cross-react with Crotonaldehyde, Hexanoyl Lysine, 4-Hydroxy-2-hexenal, 4-Hydroxy nonenal, Malondialdehyde, or Methylglyoxal modified proteins.
Target Information
Acrolein is primarily used as an intermediate in the synthesis of acrylic acid and as a biocide. It may form from the breakdown of certain pollutants in outdoor air or from the burning of organic matter including tobacco or fuels such as gasoline or oil. Acrolein is toxic to humans following inhalation, oral, or dermal exposures.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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