Thermo Fisher Scientific Acrolein Monoclonal Antibody (10A10), APC
Acrolein 변형 단백질에 특이적인 마우스 단클론 항체로, APC로 결합되어 형광 분석에 적합. Western blot, IHC, ICC, Flow cytometry, ELISA 등 다양한 응용 가능. 단백질 G로 정제된 액상 형태로 보존제 없음. 연구용으로만 사용.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (IHC) | 1:100 |
| Immunocytochemistry (ICC/IF) | 1:50 |
| Flow Cytometry (Flow) | 1:100 |
| ELISA | 1:1,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Chemical |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 10A10 |
| Immunogen | Synthetic Acrolein modified Keyhole Limpet Hemocyanin (KLH) |
| Conjugate | APC (Allophycocyanin) |
| Excitation / Emission Max | 651 / 660 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | 95.64 mM phosphate / 2.48 mM MES, pH 7.4, with 0.5 M EDTA |
| Contains | No preservative |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2932219 |
Additional Formats
- Unconjugated (Cat. MA5-27553)
- FITC (Cat. MA5-45766)
- PE (Cat. MA5-45768)
- PerCP (Cat. MA5-45767)
- Custom conjugation available upon request
Product Specific Information
A 1:1,000 dilution of MA5-45765 was sufficient for detection of Acrolein in 2 µg of Acrolein conjugated to BSA by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary antibody.
Specific for Acrolein-modified proteins. Does not detect free acrolein. Does not cross-react with Crotonaldehyde, Hexanoyl Lysine, 4-Hydroxy-2-hexenal, 4-Hydroxy nonenal, Malondialdehyde, or Methylglyoxal modified proteins.
Target Information
Acrolein is primarily used as an intermediate in the synthesis of acrylic acid and as a biocide. It may be formed from the breakdown of certain pollutants in outdoor air or from the burning of organic matter including tobacco, gasoline, or oil. Acrolein is toxic to humans following inhalation, oral, or dermal exposure.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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