Thermo Fisher Scientific Acrolein Monoclonal Antibody (2H2), PE
Acrolein에 특이적으로 반응하는 Mouse monoclonal antibody (Clone 2H2, PE conjugated). Western blot, ICC/IF, Flow cytometry, ELISA에 사용 가능. 565/576 nm 형광 특성. Protein G 정제, 보존제 무첨가, 4°C 보관.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:50 |
| Flow Cytometry (Flow) | 1:100 |
| ELISA | 1:1,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Chemical |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 2H2 |
| Immunogen | Synthetic Acrolein modified Keyhole Limpet Hemocyanin (KLH) |
| Conjugate | PE (R-Phycoerythrin) |
| Excitation / Emission Max | 565 / 576 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | 95.64 mM phosphate / 2.48 mM MES, pH 7.4, with 0.5 M EDTA |
| Contains | No preservative |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2932218 |
Additional Formats
- Unconjugated (MA5-27557)
- APC (MA5-45761)
- FITC (MA5-45762)
- PerCP (MA5-45763)
- Custom conjugation available
Product Specific Information
A 1:1,000 dilution of MA5-45764 was sufficient for detection of Acrolein in 2 µg of Acrolein conjugated to BSA by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary antibody.
Specific for Acrolein modified proteins. Does not detect free acrolein. Does not cross-react with Crotonaldehyde, Hexanoyl Lysine, 4-Hydroxy-2-hexenal, 4-Hydroxy nonenal, Malondialdehyde, or Methylglyoxal modified proteins.
Target Information
Acrolein is primarily used as an intermediate in the synthesis of acrylic acid and as a biocide. It may also be formed from the breakdown of certain pollutants in outdoor air or from the burning of organic matter such as tobacco or fuels like gasoline or oil. Acrolein is toxic to humans following inhalation, oral, or dermal exposures.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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