Thermo Fisher Scientific Acrolein Monoclonal Antibody (10A10), FITC
Acrolein 변형 단백질을 특이적으로 인식하는 FITC 결합 단클론 항체. Western blot, IHC, ICC, Flow cytometry, ELISA에 사용 가능. 마우스 IgG1 항체로, 4°C 암소 보관 권장. 다양한 형광 표지 형식으로 제공.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (IHC) | 1:100 |
| Immunocytochemistry (ICC/IF) | 1:50 |
| Flow Cytometry (Flow) | 1:100 |
| ELISA | 1:1,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Chemical |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 10A10 |
| Immunogen | Synthetic Acrolein modified Keyhole Limpet Hemocyanin (KLH) |
| Conjugate | FITC |
| Excitation / Emission Max | 498 / 517 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | 9.1 mM sodium bicarbonate/PBS, pH 7.4, with 640.91 mM DMSO, 136.36 mM ethanolamine |
| Contains | No preservative |
| Storage Conditions | 4°C, store in dark |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2932220 |
Additional Formats
- Unconjugated (MA5-27553)
- APC (MA5-45765)
- PE (MA5-45768)
- PerCP (MA5-45767)
- Request custom conjugation available
Product Specific Information
A 1:1,000 dilution of MA5-45766 was sufficient for detection of Acrolein in 2 µg of Acrolein conjugated to BSA by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary antibody.
Specific for Acrolein-modified proteins. Does not detect free acrolein. Does not cross-react with Crotonaldehyde, Hexanoyl Lysine, 4-Hydroxy-2-hexenal, 4-Hydroxy nonenal, Malondialdehyde, or Methylglyoxal modified proteins.
Target Information
Acrolein is primarily used as an intermediate in the synthesis of acrylic acid and as a biocide. It may be formed from the breakdown of certain pollutants in outdoor air or from the burning of organic matter including tobacco, or fuels such as gasoline or oil. Acrolein is toxic to humans following inhalation, oral, or dermal exposure.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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