
Thermo Fisher Scientific IL-5 Monoclonal Antibody (TRFK4), eBioscience
Mouse IL-5를 인식하는 단클론 항체로, ELISA 분석에 최적화되어 있습니다. TRFK4 클론으로 제작되었으며, Rat IgG2a 아이소타입입니다. 고순도(>90%)로 정제되어 있으며, 4°C에서 보관합니다. 연구용으로만 사용 가능합니다.
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Applications
Tested Application
- ELISA: 1 µg/mL
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Host / Isotype | Rat / IgG2a, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | TRFK4 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_468419 |
Additional Formats
Product Specific Information
Description:
The TRFK4 antibody reacts with mouse interleukin-5 (IL-5), also known as BCDF-alpha and eosinophil differentiation factor (EDF). This 15–20 kDa cytokine is secreted by Th2 cells and exists as a homodimer.
Applications Reported:
TRFK4 has been reported for use in detection of mouse IL-5 by ELISA.
Applications Tested:
The Biotin-conjugated TRFK4 has been tested by sandwich ELISA for detection of mouse IL-5 in combination with TRFK5 and can be used at 1 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
IL-5 is a cytokine that acts as a growth and differentiation factor for both B cells and eosinophils. It is a main regulator of eosinopoiesis, eosinophil maturation, and activation. Elevated IL-5 production is associated with asthma and hypereosinophilic syndromes.
The IL-5 receptor is a heterodimer whose beta subunit is shared with IL-3 and GM-CSF receptors. IL-5, IL-4, IL-13, and CSF2 form a cytokine gene cluster on chromosome 5, regulated by long-range elements over 120 kb on chromosome 5q31.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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