Thermo Fisher Scientific IL-5 Monoclonal Antibody (TRFK4), eBioscience

Applications

Tested Dilution

Publications

ELISA (ELISA)

1 µg/mL

-

Product Specifications

Species Reactivity

Mouse

Host/Isotype

Rat / IgG2a, kappa

Class

Monoclonal

Type

Antibody

Clone

TRFK4

Conjugate

Unconjugated Unconjugated Unconjugated

View additional formats

• Biotin
• Request custom conjugation

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_468419

Product Specific Information

Description: The TRFK4 antibody reacts with mouse interleukin-5 (IL-5), also known as BCDF-alpha and eosinophil differentiation factor (EDF). This 15-20 kDa cytokine is secreted by Th2 cells and exists a homodimer.

Applications Reported: TRFK4 has been reported for use in detection of mouse IL-5 by ELISA.

Applications Tested: The Biotin conjugated TRFK4 has been tested by sandwich ELISA for detection of mouse IL-5 in combination with TRFK5 and can be used at 1 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

IL-5 is a cytokine that acts as a growth and differentiation factor for both B cells and eosinophils. IL-5 cytokine is a main regulator of eosinopoiesis, eosinophil maturation and activation. The elevated production of this cytokine is reported to be related to asthma or hypereosinophilic syndromes. The receptor of IL-5 cytokine is a heterodimer, whose beta subunit is shared with the receptors for interleukine 3 (IL3) and colony stimulating factor 2 (CSF2/GM-CSF). IL-5, together with those for interleukin 4 (IL4), interleukin 13 (IL13), and CSF2, form a cytokine gene cluster on chromosome 5. IL-5, IL4, and IL13 are found to be regulated coordinately by long-range regulatory elements spread over 120 kilobases on chromosome 5q31.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.