Thermo Fisher Scientific GABRA5 Monoclonal Antibody (N415/24)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

-

Immunohistochemistry (PFA fixed) (IHC (PFA))

1:100

-

Immunocytochemistry (ICC/IF)

1:100

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

N415/24

Immunogen

Fusion protein amino acids 368-419 (last cytoplasmic domain before extracellular C-terminus) of human GABA A Receptor Alpha5. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:GABRA5,uniProtId:P31644-1,ncbiNodeId:9606,antigenRange:368-419,antigenLength:462,antigenImageFileName:MA5-27700_GABRA5_P31644-1_House_mouse.svg,antigenImageFileNamePDP:MA5-27700_GABRA5_P31644-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

View additional formats

• APC
• FITC
• PE
• PerCP
• Request custom conjugation

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

PBS, pH 7.4, with 50% glycerol

Contains

0.1% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice

RRID

AB_2735193

Product Specific Information

A 1:100 dilution of MA5-27700 was sufficient for detection of GABA-A R, Alpha5 in 20 µg of mouse brain lysate by ECL immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody. |Detects approximately 55kDa. Does not cross-react with GABA A Receptor Alpha2 or other GABA-A Receptors based on KO validation data.

This antibody was formerly sold as clone S415-24.

Target Information

GABRA5 is a part of GABA, a major inhibitory neurotransmitter in the mammalian brain where it acts at GABA-A receptors, which are ligand-gated chloride channels. Chloride conductance of these channels can be modulated by agents such as benzodiazepines that bind to the GABA-A receptor. At least 16 distinct subunits of GABA-A receptors have been identified. Transcript variants utilizing three different alternative non-coding first exons have been described.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.