Thermo Fisher Scientific GABRA5 Monoclonal Antibody (N415/24)
GABRA5 단백질을 인식하는 Mouse monoclonal antibody (Clone N415/24). Western blot, IHC, ICC/IF에 사용 가능. Human, Mouse, Rat 시료와 반응. Protein G로 정제된 액상 시약으로, -20°C에서 보관. 비특이적 교차반응 없이 높은 특이성 제공.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | 1:100 |
| Immunocytochemistry (ICC/IF) | 1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | N415/24 |
| Immunogen | Fusion protein amino acids 368–419 (last cytoplasmic domain before extracellular C-terminus) of human GABA A Receptor Alpha5 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.1% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2735193 |
Additional Formats
- APC: MA5-45745
- FITC: MA5-45746
- PE: MA5-45748
- PerCP: MA5-45747
- Custom conjugation available upon request
Product Specific Information
A 1:100 dilution of MA5-27700 was sufficient for detection of GABA-A R, Alpha5 in 20 µg of mouse brain lysate by ECL immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Detects approximately 55 kDa. Does not cross-react with GABA A Receptor Alpha2 or other GABA-A Receptors based on KO validation data.
This antibody was formerly sold as clone S415-24.
Target Information
GABRA5 is part of GABA, a major inhibitory neurotransmitter in the mammalian brain acting at GABA-A receptors, which are ligand-gated chloride channels. Chloride conductance of these channels can be modulated by agents such as benzodiazepines that bind to the GABA-A receptor.
At least 16 distinct subunits of GABA-A receptors have been identified. Transcript variants utilizing three different alternative non-coding first exons have been described.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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