Thermo Fisher Scientific GABRA1 Monoclonal Antibody (N95/35)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:1,000

-

Immunocytochemistry (ICC/IF)

1:100

-

Antibody Microarray (AM)

Assay-dependent

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

N95/35

Immunogen

Fusion protein amino acids 355-394 of human GABA-A-R-Alpha1 if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:GABRA1,uniProtId:P14867-1,ncbiNodeId:9606,antigenRange:355-394,antigenLength:456,antigenImageFileName:MA5-27703_GABRA1_P14867-1_House_mouse.svg,antigenImageFileNamePDP:MA5-27703_GABRA1_P14867-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

View additional formats

• APC
• FITC
• PE
• PerCP
• Request custom conjugation

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

PBS, pH 7.4, with 50% glycerol

Contains

0.1% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice

RRID

AB_2735190

Product Specific Information

1 µg/mL of MA5-27703 was sufficient for detection of alpha1 GABA receptor in 10 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.|Detects approximately 55kDa. No cross-reactivity against GABA-A-R-Alpha 2 or -Alpha3.

Target Information

Gamma -aminobutyric acid (GABA) is the primary inhibitory neurotransmitter in the central nervous system, causing a hyperpolarization of the membrane through the opening of a Cl- channel associated with the GABAA Receptor (GABAA-R) subtype. GABAA-Rs are important therapeutic targets for a range of sedative, anxiolytic, and hypnotic agents and are implicated in several diseases including epilepsy, anxiety, depression, and substance abuse. The GABAA-R is a multimeric subunit complex. To date aix alphas, four betas and four gammas, plus alternative splicing variants of some of these subunits, have been identified. Injection in oocytes or mammalian cell lines of cRNA coding for alpha- and beta-subunits results in the expression of functional GABAA-R sensitive to GABA. However, coexpression of a gamma-subunit is required for benzodiazepine modulation. The various effects of the benzodiazepines in brain may also be mediated via different alpha-subunits of the receptor. Lastly, phosphorylation of beta-subunits of the receptor has been shown to modulate GABAA-R function.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.