Thermo Fisher Scientific FOXP3 Monoclonal Antibody (FJK-16s), Brilliant Violet 786, eBioscience

Thermo Fisher Scientific FOXP3 Monoclonal Antibody (FJK-16s), Brilliant Violet 786, eBioscience™

Applications and Tested Dilution

Species Reactivity

Bovine, Dog, Cat, Mouse, Pig, Rat

Host / Isotype

Rat / IgG2a, kappa

Recommended Isotype Control

Rat IgG2a kappa Isotype Control (eBR2a), Brilliant Violet™ 786, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

FJK-16s

Conjugate

Brilliant Violet™ 786

Excitation / Emission Max

406 / 788 nm

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage Buffer

PBS, pH 7.2, with BSA

Contains

0.09% sodium azide

Storage Conditions

4°C, store in dark, DO NOT FREEZE

Shipping Conditions

Wet ice

Product Specific Information

The FJK-16s antibody reacts with mouse, rat, dog, porcine, bovine, and cat Foxp3 (FORKHEAD BOX P3, SCURFIN, JM2). Foxp3 is a 49–55 kDa transcriptional regulator crucial for regulatory T cell (Treg) function. The antibody epitope maps to amino acids 75–125 of the mouse Foxp3 protein.

• Optimized for use with the Foxp3/Transcription Factor Buffer Staining Set (Product #00-5523-00)
• Reported for intracellular staining and flow cytometric analysis
• Tested using mouse splenocytes with the Foxp3/Transcription Factor Staining Buffer Set
• Recommended use: ≤1.0 µg/test (100 µL final volume)

Brilliant Violet™ 786 (BV786) is a tandem dye emitting at 786 nm, for cytometers equipped with a 405 nm violet laser.

Buffer Recommendations:

• Use Super Bright Complete Staining Buffer (SB-4401-42) or Brilliant Stain Buffer™ (00-4409-75) to minimize non-specific interactions when using multiple polymer dyes.

Fixation and Storage:

• Store stained samples in IC Fixation Buffer (00-8222-49) or 1-step Fix/Lyse Solution (00-5333-54) up to 3 days at 4°C in the dark.
• Not compatible with methanol-based fixation.
• Protect from light due to photo-induced oxidation sensitivity.

Excitation: 407 nm
Emission: 786 nm
Laser: Violet Laser

BRILLIANT VIOLET™ is a trademark or registered trademark of Becton, Dickinson and Company or its affiliates, used under license. Powered by Sirigen.™

Target Information

FOXP3 (Forkhead box protein 3) is a transcriptional regulator essential for immune homeostasis.

• Expressed in CD25⁺CD4⁺ regulatory T cells
• Defects cause scurfy phenotype in mice and IPEX syndrome in humans
• Plays a role in immune regulation and autoimmune disease control
• Expression in cancer cells regulated by p53 in response to DNA damage

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Additional Formats Available

• Unconjugated
• Alexa Fluor 488, 532, 561, 660, 700
• APC, Biotin
• Brilliant Violet 421, 605, 711
• eFluor 450, 506, 570, 615, 660
• FITC, PE, PE-Cyanine5, PE-Cyanine5.5, PE-Cyanine7, PE-eFluor 610
• PerCP-Cyanine5.5, PerCP-eFluor 710
• Request custom conjugation

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