
Thermo Fisher Scientific Armenian Hamster IgG Isotype Control (eBio299Arm), Alexa Fluor 660, eBioscience
Armenian hamster IgG 동형 대조군 항체로, Alexa Fluor 660 형광 표지. 유세포 분석용 음성 대조군으로 사용 가능. 663/691 nm의 여기/방출 특성. 친화 크로마토그래피 정제, 4°C 암소 보관.
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Applications
- Flow Cytometry (Flow): Assay-Dependent
- Control (Ctrl): Assay-Dependent
Product Specifications
| 항목 | 내용 |
|---|---|
| Host/Isotype | Armenian hamster / IgG |
| Class | Monoclonal |
| Type | Isotype Control |
| Clone | eBio299Arm |
| Conjugate | Alexa Fluor™ 660 |
| Excitation/Emission Max | 663 / 691 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2896284 |
Additional Formats Available
- Unconjugated
- Alexa Fluor 488, 532, 700
- APC, APC-eFluor 780
- Biotin
- Brilliant UV 395–805
- Brilliant Violet 421–786
- eFluor 450, 506, 615, 660
- FITC
- Functional Grade
- PE, PE-Cyanine5, PE-Cyanine5.5, PE-Cyanine7, PE-eFluor 610
- PerCP-Cyanine5.5, PerCP-eFluor 710
- Super Bright 436, 600, 645, 702, 780
- Custom conjugation available
Product Specific Information
The eBio299Arm monoclonal antibody is useful as an isotype control immunoglobulin.
This antibody has been reported for use in flow cytometric analysis and tested on mouse splenocytes and normal human peripheral blood cells.
It should be used at the same concentration as the experimental antibody.
Alexa Fluor 660 emits at 690 nm and is intended for use on spectral cytometers where it may be multiplexed with APC, Alexa Fluor 647, and Alexa Fluor 700.
Excitation: 662 nm
Emission: 690 nm
Laser: Red Laser
Target Information
Isotype control antibodies serve as negative controls to differentiate non-specific background signal from specific antibody signal.
They help identify background staining caused by Fc receptor binding, non-specific interactions, or cell autofluorescence.
These controls are mainly used in flow cytometry but are also applicable in ChIP, immunohistochemistry, and gel shift assays.
Isotype controls should match the species and isotype of the primary antibody and, if directly labeled, use the same conjugate for accurate comparison.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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