Thermo Fisher Scientific GEMIN8 Monoclonal Antibody (OTI5C8), TrueMAB
GEMIN8 단백질을 표적으로 하는 Mouse IgG1 단클론 항체로, Western blot에 적합. 인간 GEMIN8 전장 재조합 단백질로 면역화됨. 무보존제, 동결건조 형태로 제공되며 -20°C에서 보관. 연구용으로 snRNP 복합체 관련 단백질 분석에 활용 가능.
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Applications
- Western Blot (WB)
Tested Dilution: 1:2,000
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI5C8 |
| Immunogen | Full length human recombinant protein of human GEMIN8 produced in E. coli |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
The SMN complex catalyzes the assembly of small nuclear ribonucleoproteins (snRNPs), essential components of the spliceosome, and plays a crucial role in pre-mRNA splicing.
Spliceosomal snRNPs contain Sm proteins (SNRPB, SNRPD1, SNRPD2, SNRPD3, SNRPE, SNRPF, SNRPG) forming a heptameric ring on small nuclear RNA.
In cytosol, Sm proteins are held in an inactive 6S pICln-Sm complex by CLNS1A. The SMN complex dissociates CLNS1A and transfers Sm proteins to the SMN-Sm complex, enabling snRNP assembly and nuclear transport.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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