Thermo Fisher Scientific ITGA4 Monoclonal Antibody (Bu49)

Applications

Tested Dilution

Publications

Immunohistochemistry (Frozen) (IHC (F))

Assay-dependent

-

Immunocytochemistry (ICC/IF)

-

View 1 publication 1 publication

Flow Cytometry (Flow)

1:50-1:200

-

Immunoprecipitation (IP)

Assay-dependent

-

Product Specifications

Species Reactivity

Dog, Human

Published species

Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

Bu49

Immunogen

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.4

Contains

0.09% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_928543

Product Specific Information

The epitope recognized by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. We recommend the use of acetone fixation for frozen sections. A suggested positive control for immunohistochemical applications is human tonsil. For FACS analysis, use 10 µL of the suggested working dilution to label 1x10^6 cells in 100 µL.

Mouse anti Human CD49d monoclonal antibody, clone Bu49 recognizes human CD49d, also known as integrin alpha 4 or VLA-4 alpha subunit. CD49d is a approximately 150 kDa single pass type I transmembrane glycoprotein.

Target Information

The integrin alpha 4 (also known as CD49d and ITGA4) belongs to the integrin alpha chain family of proteins. Integrins are heterodimeric integral membrane proteins composed of an alpha and beta chains. Alpha4 chain associates with either beta1 or beta7 chain. It has been demonstrated that the putative ligand-binding sites of both integrin alpha4beta1 and alpha4beta7 is located on the alpha4 chain. These ligands included Madcam, VCAM, and fibronectin. Madcam is known as the principal ligand for integrin alpha4beta7. Recently it was also demonstrated that HIV-1 envelope can mimic Madcam by binding to and signaling though integrin alpha4beta7, the gut mucosal homing receptor for peripheral T cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.