Thermo Fisher Scientific NGF Recombinant Rabbit Monoclonal Antibody (SI79-01)
Recombinant rabbit monoclonal antibody targeting NGF with high specificity and sensitivity. Validated for WB, IHC, and ICC/IF applications. Offers lot-to-lot consistency and animal origin-free formulation. Suitable for human, mouse, rat, and zebrafish ...
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:2,000 | View 5 publications |
| Immunohistochemistry (IHC) | - | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:100 | - |
| Immunohistochemistry (Frozen) (IHC (F)) | - | View 1 publication |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 | - |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat, Zebrafish |
| Published Species | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | SI79-01 |
| Immunogen | Synthetic peptide within Human NGF (aa 192–241) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | TBS, pH 7.4, with 40% Glycerol, 0.05% BSA |
| Contains | 0.05% Sodium Azide |
| Storage Conditions | Store at 4°C short term. For long term, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2809361 |
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems developed by cloning specific antibody DNA sequences from immunoreactive rabbits. Individual clones are screened to select optimal candidates for production.
Advantages:
- High specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulation
- Broader immunoreactivity due to larger rabbit immune repertoire
Target Information
Nerve Growth Factor (NGF) influences the survival and differentiation of various neuronal and non-neuronal cells. It binds to specific cell surface receptors with both high and low affinity sites, where only the high-affinity receptor mediates signal transduction.
Key points:
- High-affinity receptor forms a 158 kDa complex with NGF
- Low-affinity receptor forms a 100 kDa complex
- Human and rat NGF receptor genes show >90% homology
- NGF regulates growth and differentiation of sympathetic and sensory neurons
- Mutations linked to hereditary sensory and autonomic neuropathy type 5 (HSAN5)
- Dysregulation associated with allergic rhinitis
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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