Thermo Fisher Scientific Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), eFluor 660, eBioscience
Thermo Fisher Scientific의 Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), eFluor 660은 Flow Cytometry 및 Immunohistochemistry용 아이소타입 컨트롤 항체입니다. eFluor 660 형광체로 레드 레이저(633 nm)에서 여기되며, 실험 항체와 동일한 조건에서 사용 가능합니다. 연구용으로만 사용됩니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Immunohistochemistry (IHC) | Assay-Dependent |
| Flow Cytometry (Flow) | Assay-Dependent |
| Control (Ctrl) | Assay-Dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Isotype Control |
| Clone | P3.6.2.8.1 |
| Conjugate | eFluor™ 660 |
| Excitation / Emission Max | 651 / 668 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_10598653 |
Additional Formats Available
- Unconjugated
- Alexa Fluor 488, 532, 561, 647, 660, 700
- APC, APC-eFluor 780
- Biotin
- Brilliant UV Series (395–805)
- Brilliant Violet Series (421–786)
- eFluor 450, 506, 570, 615
- FITC
- Functional Grade
- Pacific Orange
- PE, PE-Cyanine5, PE-Cyanine5.5, PE-Cyanine7, PE-eFluor 610
- PerCP-Cyanine5.5, PerCP-eFluor 710
- Super Bright Series (436–780)
- Custom conjugation available upon request
Product Specific Information
The monoclonal mouse IgG1 kappa immunoglobulin serves as an isotype control.
Reported applications include flow cytometric analysis and immunohistochemical staining.
Tested by flow cytometric analysis of normal human peripheral blood cells and can be used at the same concentration as the experimental antibody.
eFluor® 660 is a replacement for Alexa Fluor® 647, emitting at 659 nm and excited with a red laser (633 nm). Ensure your instrument is capable of detecting this fluorochrome.
- Excitation: 633–647 nm
- Emission: 668 nm
- Laser: Red laser
- Filtration: 0.2 µm post-manufacturing filtered
Target Information
Mouse IgG1 is a subtype of immunoglobulin G antibody produced by B cells in response to antigens. It is the most abundant IgG subtype in serum and plays a key role in adaptive immune responses such as opsonization, complement activation, and antibody-dependent cell-mediated cytotoxicity.
It is widely used in research applications including ELISA, Western blotting, and immunohistochemistry, as well as in therapeutic research such as immunotherapy and vaccine development.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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