Thermo Fisher Scientific Phospho-JNK1/JNK2 (Thr183, Tyr185) Recombinant Rabbit Monoclonal Antibody (D12H7L17)

Applications

Tested Dilution

Publications

Western Blot (WB)

0.1-1 µg/mL

View 11 publications 11 publications

Immunohistochemistry (IHC)

-

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:10-1:100

-

Immunocytochemistry (ICC/IF)

4-6 µg/mL

-

Flow Cytometry (Flow)

0.5-5 µg/1x10^6 cells

-

ELISA (ELISA)

1-5 µg/mL

-

ChIP assay (ChIP)

1 µL

-

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human, Insect, Mouse

Host/Isotype

Rabbit / IgG

Expression System

Expi293

Class

Recombinant Monoclonal

Type

Antibody

Clone

D12H7L17

Immunogen

A peptide corresponding to amino acids 179-189 and 180-189 of P45983 and P45984, respectively. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:JNK1/JNK2,uniProtId:P45983-1,ncbiNodeId:9606,antigenRange:179-189,antigenLength:427,antigenImageFileName:700031_JNK1JNK2_P45983-1_Rabbit.svg,antigenImageFileNamePDP:700031_JNK1JNK2_P45983-1_Rabbit_PDP.jpeg,sortOrder:1},{targetFamily:JNK1/JNK2,uniProtId:P45984-1,ncbiNodeId:9606,antigenRange:180-189,antigenLength:424,antigenImageFileName:700031_JNK1JNK2_P45984-1_Rabbit.svg,antigenImageFileNamePDP:700031_JNK1JNK2_P45984-1_Rabbit_PDP.jpeg,sortOrder:2}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Protein A

Storage buffer

PBS

Contains

0.09% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2532273

Product Specific Information

This antibody is predicted to react with mouse, rat, primate, canine, bovine, porcine, chicken, equine, zebrafish and Xenopus based on sequence homology.

Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

Target Information

C-Jun N-terminal Kinase (JNK) is also known as Mitogen-activated protein kinase (MAPK8). It belongs to the MAPK superfamily of stress-activated protein kinases. MAPKs are Serine-threonine protein kinases that are activated in response to a variety of extracellular stimuli, and mediate signal transduction from the cell surface to the nucleus. JNK is involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. JNK pathways are activated by stress and inflammatory signals. JNK is expressed as ten different isoforms due to differential mRNA splicing. The predominant forms are JNK1 and JNK2.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.