Thermo Fisher Scientific IRAK4 Recombinant Rabbit Monoclonal Antibody (12H2L6)

Applications

Tested Dilution

Publications

Western Blot (WB)

2-3 µg/mL

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Immunohistochemistry (Paraffin) (IHC (P))

1:10-1:50

-

Immunocytochemistry (ICC/IF)

4-6 µg/mL

-

ChIP assay (ChIP)

1 µL

-

in situ PLA (PLA)

-

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In vitro Assay (IV)

-

- -

Product Specifications

Species Reactivity

Human

Published species

Mouse

Host/Isotype

Rabbit / IgG

Expression System

Expi293

Class

Recombinant Monoclonal

Type

Antibody

Clone

12H2L6

Immunogen

A peptide corresponding to amino acids 41-52 of Q9NWZ3. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:IRAK4,uniProtId:Q9NWZ3-1,ncbiNodeId:9606,antigenRange:41-52,antigenLength:460,antigenImageFileName:700026_IRAK4_Q9NWZ3-1_Rabbit.svg,antigenImageFileNamePDP:700026_IRAK4_Q9NWZ3-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Protein A

Storage buffer

PBS

Contains

0.09% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2532271

Product Specific Information

This antibody is predicted to react with mouse, rat, primate, ovine, equine, porcine, bovine, canine and Xenopus based on sequence homology.

Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

Target Information

IL-1 receptor associated kinases (IRAKs) are serine/threonine kinases involved in signaling via Toll/IL-1 pathways. Overexpression of IRAK-4 activates NF-kappa-B and MAPK pathways. IRAK-4 interacts with and phosphorylates IRAK-1. IRAK-4-deficient animals are completely resistant to the challenge with LPS. Animals and humans lacking IRAK-4 are impaired in their responses to viral and bacterial challenges. Members in IRAK/Pelle family play a central role in IL-1R/TLR mediated inflammatory responses and in innate immunity.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.