Thermo Fisher Scientific Phospho-JAK1 (Tyr1022, Tyr1023) Recombinant Rabbit Monoclonal Antibody (59H4L5)
Phospho-JAK1 (Tyr1022, Tyr1023) 항체로 인간 및 마우스에 반응하며, WB·ICC·ChIP 등에 적합. 재조합 토끼 단일클론으로 높은 특이성과 일관성 제공. 단기 4°C, 장기 -20°C 보관 권장.
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Applications
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 0.5–2 µg/mL | View 1 publication |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 | - |
| ChIP assay (ChIP) | 1 µL | - |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Published Species | Rat |
| Host / Isotype | Rabbit / IgG |
| Expression System | Expi293 |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | 59H4L5 |
| Immunogen | A peptide corresponding to amino acids 1030–1039 of P23458 (commonly referred to as pY1022/pY1023). |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS |
| Contains | 0.09% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2532272 |
Product Specific Information
This antibody is predicted to react with mouse, rat, chimpanzee, Rhesus monkey, bovine, canine, porcine, equine, Xenopus, and zebrafish based on sequence homology.
Intact IgG appears on a non-reducing gel as ~150 kDa band, and upon reduction generates a ~25 kDa light chain band and a ~50 kDa heavy chain.
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning specific antibody DNA sequences from immunoreactive rabbits. Individual clones are screened to select the best candidates for production.
Advantages of recombinant rabbit monoclonal antibodies:
- Superior specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulation
- Broader immunoreactivity due to larger rabbit immune repertoire
Target Information
Janus kinase 1 (JAK1) is a protein tyrosine kinase involved in interferon-alpha/beta and -gamma signal transduction pathways. Immunological stimuli such as interferons and cytokines induce recruitment of Stat transcription factors to cytokine receptor-associated JAK1. JAK1 phosphorylates proximal Stat factors, which then dimerize, translocate to the nucleus, and regulate transcription. Phosphorylation at tyrosine residues 1022 and 1023 is critical for catalytic activation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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