Thermo Fisher Scientific Cyclin B1 Monoclonal Antibody (GNS11)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:100-1:1,000

View 4 publications 4 publications

Immunohistochemistry (IHC)

-

View 3 publications 3 publications

Immunohistochemistry (Paraffin) (IHC (P))

2-4 µg/mL

-

Immunocytochemistry (ICC/IF)

1:10-1:200

View 4 publications 4 publications

Immunoprecipitation (IP)

2 µg/mL

View 1 publication 1 publication

Product Specifications

Species Reactivity

Hamster, Human, Mouse, Rat

Published species

Human, Mouse, Rat

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

GNS11

Immunogen

Human cyclin B1 protein

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.2 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.4, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10980440

Product Specific Information

MA5-14327 targets Cyclin B1 in WB, ICC/IF, IHC (P), and IP applications and shows reactivity with Hamster, Human, mouse, and Rat samples. This antibody is not recommended for NIH-3T3 cells in Western blot applications.

The MA5-14327 immunogen is human cyclin B1 protein.

This antibody was orginally validated as part of a Thermo Scientific Cellomics High Content Screening Kit. The antibody sold separately may have slightly different performance and may need to be further optimized for the best results.

Target Information

Cyclins bind to and regulate the activity of the Cyclin dependent protein kinases (CDKs). Cyclin B1 or CCNB1 is a regulatory protein involved in mitosis. It is essential for the control of the cell cycle at the G2/M transition. Cyclin B1 complexes with p34 (cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase. Cyclin B1 is not ubiquitinated during G2/M phase, resulting in its steady accumulation during G2 phase, followed by abrupt APC dependent destruction at the end of mitosis. Destruction of Cyclin B1 is required for cell cycle progression. Cyclin B1 is overexpressed in various cancers, including breast, prostate, and non-small cell lung cancer.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.