Thermo Fisher Scientific Complement C3b Monoclonal Antibody (10C7)

Applications

Western Blot (WB)

• Tested Dilution: -
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Flow Cytometry (Flow)

• Tested Dilution: 1 µg/1 million cells
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ELISA

• Tested Dilution: Assay-dependent
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Product Specifications

Product Specific Information

MA1-70054 detects C3/C3b/iC3b in human and mouse samples.
It has been successfully used in flow cytometry and ELISA procedures.
The immunogen is C57BL/6 thymocytes incubated with a rat IgG2b anti-mouse Thy-1 (RmT1) and C57BL/6 serum (as a source for C3).

Target Information

Inactive complement component 3b (iC3b) is a 42 kDa neoantigen present in blood serum.
Cleavage of C3b into iC3b by the protease factor I, in the presence of cofactors, changes the structure and resulting binding properties, preventing binding by complement factor B and properdin.
This prevents further progression of the complement pathway, providing another mechanism by which the complement pathway may be regulated.

iC3b is thought to play a role in inducing tolerance, binding complement receptor type 3 on antigen-presenting cells and stimulating them to produce transforming growth factor beta2 and interleukin-10.
It is also thought to stimulate B-cells via the CD21 receptor, providing a link between the innate and adaptive immune responses.
Additionally, iC3b acts as an opsonin recognized by complement receptor 3 on leukocytes or CRIg on Kupffer cells, stimulating phagocytosis of tagged cells.
Elevated iC3b levels are often observed in diseases such as Systemic Lupus Erythematosus and Rheumatoid Arthritis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.