Thermo Fisher Scientific CD25 Monoclonal Antibody (eBio3C7 (3C7)), Alexa Fluor 488, eBioscience

Thermo Fisher Scientific CD25 Monoclonal Antibody (eBio3C7 (3C7)), Alexa Fluor 488, eBioscience

Applications

• Flow Cytometry (Flow)

Tested Dilution: 0.06 µg/test
Publications: View 5 publications

Product Specifications

Additional Formats

• eFluor 450
• Functional Grade
• Request custom conjugation

Product Specific Information

The eBio3C7 antibody reacts with mouse CD25, the 55 kDa interleukin-2 receptor alpha chain (IL-2Ra).
CD25 is expressed by early progenitors of the T and B lineage as well as by activated mature T and B lymphocytes.
By itself, CD25 binds IL-2 only with low affinity; however, it associates with CD122 (IL-2 receptor beta chain) and CD132 (common gamma chain) to form the high-affinity IL-2 receptor.

Binding of IL-2 to both the high and low affinity classes of the IL-2 receptor is inhibited by the 3C7 antibody.
CD25 plays a role in lymphocyte differentiation and activation/proliferation.
The epitopes recognized by eBio3C7, PC61.5, and eBio7D4 are all different, allowing for depletion with one antibody (typically PC61.5) and subsequent detection with another CD25 antibody.

Applications Reported:
This eBio3C7 (3C7) antibody has been reported for use in flow cytometric analysis.

Applications Tested:
Tested by flow cytometric analysis of mouse splenocytes. Recommended use ≤ 0.06 µg per test (100 µL final volume).
Cell number can range from 10⁵ to 10⁸ cells/test. Titrate for optimal performance.

Excitation: 488 nm
Emission: 519 nm
Laser: Blue Laser
Filtration: 0.2 µm post-manufacturing filtered

Target Information

CD25 (IL2 receptor alpha chain/IL2RA) is a cytokine receptor involved in proliferation of T and B lymphocytes.
The receptor (IL2RA) forms a heterotrimeric complex with IL2RB and IL2RG, constituting the high-affinity IL2 receptor.
Homodimeric IL2RA chains result in low-affinity receptor, while homodimeric IL2RB chains produce medium affinity.
IL2 is primarily produced by mature T cells and acts as a growth, differentiation, and cell death regulator.
Mutations in this gene are associated with interleukin 2 receptor alpha deficiency.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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