Thermo Fisher Scientific Phospho-FLT3 (Tyr591) Polyclonal Antibody
Phospho-FLT3 (Tyr591) 폴리클로날 항체로, 인산화된 FLT3 단백질을 특이적으로 검출합니다. Western blot, IHC, ICC/IF 등 다양한 응용에 적합하며, Rabbit IgG 기반으로 제조되었습니다. 세포 신호전달 및 백혈병 연구에 유용합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:3,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Non-human primate, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthesized peptide derived from human FLT3 (Accession P36888), corresponding to amino acid residues around phosphorylated Tyr591 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Sequential chromatography |
| Storage buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage conditions | −20°C |
| Shipping conditions | Wet ice |
| RRID | AB_2817283 |
Product Specific Information
Antibody detects endogenous levels of Flt3 / CD135 only when phosphorylated at Tyr591.
Target Information
FLT3 is a tyrosine protein kinase belonging to the growth factor receptor tyrosine kinase family. Activation occurs via binding of the fms-related tyrosine kinase 3 ligand to the extracellular domain, inducing receptor homodimerization and autophosphorylation. The activated receptor phosphorylates and activates multiple cytoplasmic effectors involved in apoptosis, proliferation, and differentiation of hematopoietic cells in bone marrow. Mutations causing constitutive activation of this receptor are associated with acute myeloid leukemia and acute lymphoblastic leukemia.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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