Thermo Fisher Scientific CD38 Monoclonal Antibody (90), Brilliant Ultra Violet 395, eBioscience

Applications

Tested Dilution

• Flow Cytometry (Flow): 0.25 µg/test

Product Specifications

Additional Formats

• Unconjugated
• Alexa Fluor 700
• APC
• APC-eFluor 780
• Biotin
• Brilliant Violet 480
• Brilliant Violet 605
• eFluor 450
• FITC
• Functional Grade
• PE
• PE-Cyanine5
• PE-Cyanine7
• PE-eFluor 610
• PerCP-eFluor 710
• Request custom conjugation

Product Specific Information

The 90 monoclonal antibody reacts with the mouse CD38 molecule (~42 kDa type II transmembrane protein). CD38 is expressed at increasing levels on B cells during differentiation and down-regulated on germinal center B cells and plasma cells. It is also expressed on thymocytes, mature T cells, and NK cells.

Crosslinking of CD38 on resting B cells induces proliferation, enhanced by IL-4 and LPS co-signals. CD38 acts as a counter-receptor for CD31 and functions as an ectoenzyme with cyclase and hydrolase activity, playing roles in lymphocyte activation and differentiation.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Tested on mouse splenocytes at ≤0.25 µg/test (100 µL final volume, 10⁵–10⁸ cells/test).
• Fluorochrome: Brilliant Ultra Violet™ 395 (BUV395), excitation 350 nm, emission 395 nm, for UV (355 nm) laser-equipped cytometers.

When using multiple polymer dye-conjugated antibodies, use Super Bright Complete Staining Buffer (SB-4401-42) or Brilliant Stain Buffer™ (00-4409-75) to minimize non-specific interactions.

BRILLIANT ULTRA VIOLET™ is a trademark of Becton, Dickinson and Company, used under license. Powered by Sirigen™.

Target Information

CD38 (NAD⁺ glycohydrolase) is a type II transmembrane glycoprotein involved in activation, proliferation, and differentiation of lymphocytes, and apoptosis of progenitor cells. It functions as a multi-catalytic ectoenzyme (ADP-ribosyl cyclase, cyclic ADP-ribose hydrolase, NAD⁺ glycohydrolase) and as a receptor.

Antibodies to CD38 are used for:

• Subtyping lymphomas and leukemias
• Detecting plasma cells (e.g., myeloma identification)
• Marking activated B and T cells

CD38 is highly expressed in pancreas, liver, kidney, malignant lymphoma, and neuroblastoma. Dysfunction is associated with chronic lymphocytic leukemia and Richter's Syndrome.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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