Thermo Fisher Scientific IgM Monoclonal Antibody (II/41), PE, eBioscience
마우스 IgM 무거운 사슬에 특이적으로 반응하는 II/41 클론 단클론 항체로, PE 형광 표지되어 있습니다. Flow cytometry 등 면역분석에 적합하며 높은 특이성과 재현성을 제공합니다. 0.125 µg/test로 사용 가능하며 연구용으로만 제공됩니다.
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Thermo Fisher Scientific IgM Monoclonal Antibody (II/41), PE, eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | - | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | - | View 1 publication |
| Flow Cytometry (Flow) | 0.125 µg/test | View 59 publications |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Fish, Human, Mouse, Rat |
| Host/Isotype | Rat / IgG2a, kappa |
| Recommended Isotype Control | Rat IgG2a kappa Isotype Control (eBR2a), PE, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | II/41 |
| Conjugate | PE |
| Excitation/Emission Max | 565 / 576 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_465939 |
Available Formats
- Unconjugated
- APC
- APC-eFluor 780
- Biotin
- Brilliant Violet 605
- Brilliant Violet 711
- eFluor 450
- eFluor 570
- eFluor 660
- FITC
- NovaFluor Red 710
- NovaFluor Yellow 610
- PE-Cyanine5
- PE-Cyanine7
- PE-eFluor 610
- PerCP-eFluor 710
- Super Bright 436
- Super Bright 600
- Super Bright 645
- Super Bright 780
- Request custom conjugation
Product Specific Information
The II/41 monoclonal antibody reacts with the mu heavy chain of mouse IgM and does not react with other classes of mouse immunoglobulin (IgD, IgG, IgA). IgM is expressed intracellularly during early B cell development and on the surface of mature B cells. Fluorochrome-conjugated II/41 can be used as a detection secondary for mouse IgM.
Applications Reported: Flow cytometric analysis.
Applications Tested: Tested by flow cytometric analysis of mouse bone marrow cells. Recommended ≤0.125 µg/test (100 µL final volume). Cell number can range from 10⁵–10⁸ cells/test. Optimal titration recommended.
Excitation: 488–561 nm
Emission: 578 nm
Laser: Blue, Green, Yellow-Green
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
Mouse IgM is an immunoglobulin M antibody produced by B cells in response to antigens. It is the first antibody generated during an immune response and the largest immunoglobulin in serum. Composed of five heavy and five light chains, it plays a key role in complement activation and antigen agglutination. Widely used in research and diagnostics such as ELISA, Western blotting, and immunohistochemistry.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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