Thermo Fisher Scientific Phospho-c-Raf (Ser621) Polyclonal Antibody
Phospho-c-Raf (Ser621) 폴리클로날 항체로 인간, 쥐에 반응하며 Western blot 및 Immunoprecipitation에 적합. 항원 친화 크로마토그래피로 정제된 액상형 항체. 세포 신호전달 연구용으로 안정적이며 -20°C 보관 가능.
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Applications
Western Blot (WB)
- Tested Dilution: 1:1,000
- Publications: View 4 publications
Immunoprecipitation (IP)
- Tested Dilution: Assay-dependent
- Publications: View 1 publication
Miscellaneous PubMed (Misc)
- Tested Dilution: –
- Publications: View 1 publication
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Human, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | The antiserum was produced against a chemically synthesized phosphopeptide derived from a region of human c-Raf that contains serine 621. The sequence is conserved in mouse and rat. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification | Antigen affinity chromatography |
| Storage Buffer | Dulbecco’s PBS, pH 7.3, with 1 mg/mL BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2533670 |
Target Information
Raf-1 (c-Raf) is a MAP kinase kinase kinase (MAP3K) functioning downstream of Ras family GTPases. It directly binds Ras and phosphorylates MEK1 and MEK2, which activate ERK1 and ERK2. Activated ERKs regulate gene expression, cell cycle, apoptosis, differentiation, and migration. Mutations in this gene are associated with Noonan syndrome 5 and LEOPARD syndrome 2.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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