Thermo Fisher Scientific Phospho-c-Kit (Tyr823) Polyclonal Antibody

Applications and Tested Dilution

Product Specifications

Target Information

KIT (c-KIT) is a proto-oncogene and a type 3 transmembrane receptor for MGF (mast cell growth factor, also known as stem cell factor). KIT was first identified as the cellular homolog of the feline sarcoma viral oncogene v-kit. KIT and its ligand regulate growth and activation of various hemopoietic and non-hemopoietic cells.

Mutations in KIT are associated with:

• Gastrointestinal stromal tumors
• Mast cell disease
• Acute myelogenous leukemia
• Piebaldism

Deregulation of KIT receptor TK by the activation loop mutation D816V is a focal point in therapeutic strategies against neoplastic mast cell growth.
c-Kit is expressed in hematopoietic stem cells, germ cells, mast cells, and gastrointestinal tract cajal cells. Upon binding of stem cell factor (SCF), c-kit dimerizes, leading to receptor activation and autophosphorylation of tyrosine residues, including Tyr703. This enables docking of Grb2 and activation of the Ras/ERK signaling pathway. SCF/c-kit also activates PI3K, PLC-γ, and JAK/STAT pathways, essential for hematopoiesis, stem cell maintenance, and gametogenesis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.