Thermo Fisher Scientific Phospho-c-Kit (Tyr730) Polyclonal Antibody

Applications

Western Blot (WB)

• Tested Dilution: 1:1,000
• Publications: View 3 publications

Immunohistochemistry (Paraffin) (IHC (P))

• Tested Dilution: 1:10–1:50
• Publications: View references

Product Specifications

Target Information

KIT (c-KIT) is a proto-oncogene and a type 3 transmembrane receptor for MGF (mast cell growth factor, also known as stem cell factor). KIT was first identified as the cellular homolog of the feline sarcoma viral oncogene v-kit. KIT, together with its ligand, regulates the growth and activation of various hematopoietic and non-hematopoietic cells.

Mutations in KIT are associated with:

• Gastrointestinal stromal tumors
• Mast cell disease
• Acute myelogenous leukemia
• Piebaldism

Deregulation of the KIT receptor TK by the prevalent activation loop mutation D816V has become a focal point in therapeutic strategies aimed at curbing neoplastic mast cell growth. c-Kit is expressed in hematopoietic stem cells, germ cells, mast cells, and gastrointestinal tract Cajal cells.

Upon binding to its ligand (SCF), c-Kit dimerizes, resulting in receptor activation and autophosphorylation of tyrosine residues, including Tyr703 on the cytoplasmic domain. This modification enables docking of Grb2 and activation of the Ras/ERK signaling pathway. SCF/c-Kit can activate multiple downstream signaling pathways such as PI3K, PLC-γ, and JAK/STAT.

c-Kit receptor activation is essential for hematopoiesis, stem cell maintenance, and gametogenesis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.