Thermo Fisher Scientific SIN3A Polyclonal Antibody
SIN3A 단백질을 인식하는 Rabbit Polyclonal 항체로 Western Blot, ICC/IF, ELISA, IP 등에 사용 가능. Human, Mouse, Rat 시료 반응. Affinity Chromatography로 정제된 액상 항체로, -20°C에서 보관. 핵 및 핵소체에서 발현되는 SIN3A 단백질 검출에 적합.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:100–1:500 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| ELISA | 1 µg/mL |
| Immunoprecipitation (IP) | 0.5 µg–4 µg antibody for 200 µg–400 µg extracts |
Product Specifications
| Category | Specification |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthetic peptide corresponding to a sequence within amino acids 1–120 of human Sin3A (NP_056292.1) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.51 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.3, with 50% glycerol |
| Contains | 0.05% ProClin 300 |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2806178 |
Product Specific Information
- Positive Samples: HeLa, COS7, U-937, MCF-7, 293T, Mouse spleen
- Cellular Location: Nucleus, nucleolus
Immunogen Sequence:
MKRRLDDQES PVYAAQQRRI PGSTEAFPHQ HRVLAPAPPV YEAVSETMQS ATGIQYSVTP SYQVSAMPQS SGSHGPAIAA VHSSHHHPTA VQPHGGQVVQ SHAHPAPPVA PVQGQQQFQR
Target Information
Mammalian Sin3 (mSin3) is a 150 kDa protein closely related to the yeast SIN3 repressor protein involved in the transcriptional repression of many genes. There are two mammalian isoforms, mSin3A and mSin3B. Containing four paired amphipathic helix (PAH) domains, mSin3A and mSin3B interact with several transcriptional repressor proteins including HDAC1, HDAC2, RbAp46, MeCP2, Mad/Max heterodimer, SMRT, and N-CoR.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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