Thermo Fisher Scientific TIP47 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

-

ELISA (ELISA)

1 µg/mL

-

Product Specifications

Species Reactivity

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 135-434 of human PLIN3 (NP_0058083) if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:TIP47,uniProtId:O60664-1,ncbiNodeId:9606,antigenRange:135-434,antigenLength:434,antigenImageFileName:PA5-90695_TIP47_O60664-1_Rabbit.svg,antigenImageFileNamePDP:PA5-90695_TIP47_O60664-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.12 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.01% thimerosal

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2806182

Product Specific Information

Immunogen sequence: MVSSAKDTVA TQLSEAVDAT RGAVQSGVDK TKSVVTGGVQ SVMGSRLGQM VLSGVDTVLG KSEEWADNHL PLTDAELARI ATSLDGFDVA SVQQQRQEQS YFVRLGSLSE RLRQHAYEHS LGKLRATKQR AQEALLQLSQ VLSLMETVKQ GVDQKLVEGQ EKLHQMWLSW NQKQLQGPEK EPPKPEQVES RALTMFRDIA QQLQATCTSL GSSIQGLPTN VKDQVQQARR QVEDLQATFS SIHSFQDLSS SILAQSRERV ASAREALDHM VEYVAQNTPV TWLVGPFAPG ITEKAPEEKK; Positive Samples: HeLa, Jurkat, SW480, 293T; Cellular Location: Cytoplasm, Cytoplasmic side, Endosome membrane, Lipid droplet, Peripheral membrane protein

Target Information

TIP47 has been described as cargo protein involved in the trafficking of the mannose-6-phosphate receptor between endosomes and the Golgi complex. Mannose 6-phophate receptors (MPRs) deliver lysosomal hydrolase from the Golgi to endosomes and then return to the Golgi complex. The protein encoded by this gene interacts with the cytoplasmic domains of both cation-independent and cation-dependent MPRs and is required for endosome to Golgi transport. This protein also binds directly to the GTPase RAB9, a member of the RAS oncogene family. The interaction with RAB9 has been shown to increase the affinity of this protein for its cargo. TIP47 has been localized in milk fat globule membranes of human and bovine origin. It has been described as a placental protein, as well. Increased amounts of TIP47 are secreted into circulation of cervix carcinoma patients. Therefore, this protein is probably significant as an oncodevelopmental marker.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.