Thermo Fisher Scientific CD103 (Integrin alpha E) Monoclonal Antibody (B-Ly7), NovaFluor Red 685, eBioscience

Thermo Fisher Scientific CD103 (Integrin alpha E) Monoclonal Antibody (B-Ly7), NovaFluor Red 685

Applications

• Flow Cytometry (Flow)

Tested Dilution: 5 µL (0.4 µg)/test

Product Specifications

Additional Formats

• Unconjugated
• APC
• FITC
• NovaFluor Blue 610-70S
• NovaFluor Blue 660-120S
• NovaFluor Yellow 610
• NovaFluor Yellow 730
• PE
• PE-Cyanine7
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 600
• Super Bright 780
• Request custom conjugation

Product Specific Information

The B-Ly7 monoclonal antibody reacts with human CD103, the alpha E integrin. CD103 non-covalently associates with integrin beta 7 and is mainly expressed on intraepithelial lymphocytes and a small subset of peripheral lymphocytes. It is also expressed by hairy cell leukemia (HCL) and some chronic B cell lymphocytic leukemias. In vitro stimulation of human T cells with mitogens induces upregulation of CD103. E-cadherin binds to CD103 and mediates homing of lymphocytes to the intestinal epithelium.

Each product contains:

• 1 vial of NovaFluor conjugate
• 1 vial of CellBlox Plus Blocking Buffer

Applications Reported: Flow cytometric analysis
Applications Tested: Pre-titrated and tested by flow cytometric analysis of 2–3 day PHA-activated human peripheral blood cells. Recommended use: 5 µL (0.4 µg) per test (100 µL final volume).

Note: NovaFluor dyes are not compatible with DNA intercalating viability dyes (e.g., propidium iodide, 7-AAD, DAPI). Use Invitrogen LIVE/DEAD Fixable Dead Cell stains instead.

This conjugate ships with CellBlox Plus Blocking Buffer (Cat. No. C001T06F01), which provides improved staining performance. Add 5 µL per sample to antibody cocktails prior to combining with cells. For single-color controls, use 5 µL per 100 µL cell sample.

NovaFluor conjugates utilize Phiton™ technology with engineered fluorescent signatures to minimize spillover and spread.

Excitation: 637 nm
Emission: 685 nm
Laser: 633–640 nm (Red)

Target Information

CD103 (ITGAE, Integrin alpha E) is expressed on mucosa-associated T lymphocytes and activated cells. It consists of a 150 kDa alpha chain and a 120 kDa beta chain. CD103 is expressed by 0.5–2% of resting lymphocytes in peripheral blood and lymphoid organs, and stains a few bone marrow cells. It forms a complex with beta 7 integrin to bind E-cadherin, mediating adhesion and activation of intestinal intraepithelial lymphocytes (IEL).
Diseases associated with CD103 dysfunction include hairy cell leukemia and splenic marginal zone lymphoma.

For Research Use Only. Not for use in diagnostic procedures.

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