Thermo Fisher Scientific CD103 (Integrin alpha E) Monoclonal Antibody (B-Ly7), NovaFluor Blue 660-120S, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 5 µL (0.6 µg)/test

Product Specifications

Additional Formats

• Unconjugated
• APC
• FITC
• NovaFluor Blue 610-70S
• NovaFluor Red 685
• NovaFluor Yellow 610
• NovaFluor Yellow 730
• PE
• PE-Cyanine7
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 600
• Super Bright 780
• Request custom conjugation

Product Specific Information

Description:
The B-Ly7 monoclonal antibody reacts with human CD103 (Integrin αE), which associates non-covalently with integrin β7. CD103 is mainly expressed on intraepithelial lymphocytes and a small subset of peripheral lymphocytes. It is also found in hairy cell leukemia (HCL) and some chronic B cell lymphocytic leukemias. E-cadherin binds to CD103 and mediates lymphocyte homing to the intestinal epithelium.

Each product includes 1 vial of NovaFluor conjugate and 1 vial of CellBlox Plus Blocking Buffer.

Applications Reported:
B-Ly7 has been reported for use in flow cytometric analysis.

Applications Tested:
This antibody has been pre-titrated and tested by flow cytometric analysis of 2–3 day PHA-activated human peripheral blood cells.

• Recommended use: 5 µL (0.6 µg) per test
• A test = antibody amount to stain a cell sample in 100 µL final volume
• Cell number: 10⁵–10⁸ cells/test (empirical determination)

Important Notes:

• NovaFluor dyes are not compatible with DNA intercalating viability dyes (e.g., propidium iodide, 7-AAD, DAPI).
• Use Invitrogen LIVE/DEAD Fixable Dead Cell stains for viability assessment.
• CellBlox Plus Blocking Buffer (Cat. No. C001T06F01) is required for optimal staining.
• Add 5 µL per sample to antibody cocktails prior to combining with cells.
• For single-color controls: use 5 µL of CellBlox Plus Blocking Buffer per 100 µL sample (10³–10⁸ cells).

NovaFluor Technology:
NovaFluor conjugates are based on Phiton™ technology utilizing novel nucleic acid dye structures engineered for optimal fluorescent signatures with minimal spillover and spread.

• Excitation: 509 nm
• Emission: 665 nm
• Laser: 488 nm (Blue)

Target Information

CD103 (ITGAE, Integrin αE):
A molecule expressed on mucosa-associated and activated T lymphocytes, comprising a 150 kDa α-chain and a 120 kDa β-chain. It is expressed by 0.5–2% of resting lymphocytes in peripheral blood and lymphoid organs, and rarely in bone marrow or peripheral blood lymphocytes.
CD103 is part of the integrin family, forming the E-cadherin binding integrin (human mucosal lymphocyte-1 antigen) when combined with β7 integrin. It plays roles in adhesion and activation of intestinal intraepithelial lymphocytes (IEL).
Associated diseases include hairy cell leukemia and splenic marginal zone lymphoma.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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