Thermo Fisher Scientific CD103 (Integrin alpha E) Monoclonal Antibody (B-Ly7), NovaFluor Yellow 610, eBioscience

Thermo Fisher Scientific CD103 (Integrin alpha E) Monoclonal Antibody (B-Ly7), NovaFluor Yellow 610, eBioscience

Applications

• Flow Cytometry (Flow)

Tested Dilution: 5 µL (0.6 µg)/test

Product Specifications

Additional Formats

• Unconjugated
• APC
• FITC
• NovaFluor Blue 610-70S
• NovaFluor Blue 660-120S
• NovaFluor Red 685
• NovaFluor Yellow 730
• PE
• PE-Cyanine7
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 600
• Super Bright 780
• Request custom conjugation

Product Specific Information

Description:
The B-Ly7 monoclonal antibody reacts with human CD103, the alpha E integrin. CD103 non-covalently associates with integrin beta 7 and is mainly expressed on intraepithelial lymphocytes and a small subset of peripheral lymphocytes. It is also expressed in hairy cell leukemia (HCL) and some chronic B cell lymphocytic leukemias. In vitro stimulation of human T cells with mitogens induces upregulation of CD103. E-cadherin binds to CD103 and mediates homing of lymphocytes to the intestinal epithelium.

Each product contains:

• 1 vial of NovaFluor conjugate
• 1 vial of CellBlox Plus Blocking Buffer

Applications Reported:
B-Ly7 has been reported for use in flow cytometric analysis.

Applications Tested:
Pre-titrated and tested by flow cytometric analysis of 2–3 day PHA-activated human peripheral blood cells. Use at 5 µL (0.6 µg) per test (100 µL final volume, 10⁵–10⁸ cells/test).

Compatibility Notes:
NovaFluor dyes are not compatible with DNA intercalating viability dyes (e.g., propidium iodide, 7-AAD, DAPI).
Use Invitrogen LIVE/DEAD Fixable Dead Cell stains for NovaFluor dyes.

CellBlox Plus Blocking Buffer:
Updated conjugate ships with CellBlox Plus Blocking Buffer (Cat. No. C001T06F01), required for optimal staining. Add 5 µL per sample before combining with cells. For single-color controls, use 5 µL per 100 µL cell sample (10³–10⁸ cells).

Technology:
NovaFluor conjugates are based on Phiton™ technology utilizing novel nucleic acid dye structures engineered for optimized fluorescent signatures, minimizing spillover and spread.

Optical Properties:
Excitation: 552 nm
Emission: 612 nm
Laser: 561 nm (Yellow)

Target Information

CD103 (ITGAE, Integrin alpha E) is expressed on mucosa-associated T lymphocytes and activated cells. It includes a 150 kDa alpha chain and a 120 kDa beta chain. Expressed by 0.5–2% of resting lymphocytes in peripheral blood and lymphoid organs. CD103 antibody stains some bone marrow cells and rare peripheral blood lymphocytes. As part of the integrin family, CD103 forms a heterodimer with beta 7 integrin, creating the E-cadherin binding integrin known as human mucosal lymphocyte-1 antigen. Preferentially expressed in intestinal intraepithelial lymphocytes (IEL), CD103 plays roles in adhesion and activation.
Diseases associated with CD103 dysfunction include hairy cell leukemia and splenic marginal zone lymphoma.

For Research Use Only.
Not for use in diagnostic procedures or resale without authorization.

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