Thermo Fisher Scientific CD25 Monoclonal Antibody (BC96), Alexa Fluor 660, eBioscience

Thermo Fisher Scientific CD25 Monoclonal Antibody (BC96), Alexa Fluor 660, eBioscience

Applications

• Flow Cytometry (Flow)

Tested Dilution: 5 µL (0.125 µg)/test
Publications: - References Component ID

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 488
• Alexa Fluor 532
• Alexa Fluor 647
• Alexa Fluor 700
• APC
• APC-eFluor 780
• Biotin
• Brilliant UV 615 / 737 / 805
• Brilliant Violet 421 / 711
• eFluor 450
• FITC
• NovaFluor Red 685 / 700
• NovaFluor Yellow 700
• PE / PE-Cyanine5 / PE-Cyanine5.5 / PE-Cyanine7
• PE-eFluor 610
• PerCP-Cyanine5.5 / PerCP-eFluor 710
• Super Bright 436 / 600 / 645 / 702 / 780
• Request custom conjugation

Product Specific Information

The BC96 monoclonal antibody reacts with human CD25, the 55 kDa interleukin-2 receptor alpha chain (IL-2Rα). CD25 is expressed by early progenitors of T and B lineage as well as by activated mature T and B lymphocytes. By itself, CD25 binds IL-2 only with low affinity. However, CD25 associates with CD122 (IL-2 receptor beta chain) and CD132 (common gamma chain) to form the high-affinity IL-2 receptor. CD25 plays a role in lymphocyte differentiation and activation/proliferation.

Applications Reported: Flow cytometric analysis
Applications Tested: Pre-diluted and tested on normal human peripheral blood cells. Recommended use at 5 µL (0.125 µg) per test (final volume 100 µL). Cell number range: 10⁵–10⁸ cells/test.

Fluorophore Characteristics:
Alexa Fluor 660 emits at 690 nm and is intended for use on spectral cytometers where it can be multiplexed with APC, Alexa Fluor 647, and Alexa Fluor 700.
Excitation: 662 nm | Emission: 690 nm | Laser: Red Laser

Target Information

CD25 (IL2 receptor alpha chain/IL2RA) is a cytokine receptor involved in T and B lymphocyte proliferation. The IL2RA receptor complex consists of IL2RA, IL2RB, and IL2RG, forming a high-affinity IL-2 receptor. Homodimeric IL2RA chains yield low-affinity receptors, while IL2RB homodimers yield medium-affinity receptors. IL2 expression in mature thymocytes is monoallelic, regulating gene expression precisely. IL2 acts as a growth and differentiation factor and regulates cell death. It stimulates B cell proliferation, enhances NK cell activity, and inhibits granulocyte macrophage colony formation. Mutations in IL2RA are associated with interleukin-2 receptor alpha deficiency.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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