Thermo Fisher Scientific CD45R (B220) Monoclonal Antibody (RA3-6B2), Alexa Fluor 660, eBioscience
Alexa Fluor 660으로 표지된 RA3-6B2 클론의 CD45R(B220) 단클론 항체로, 마우스 및 인간 시료의 B세포 표지에 적합합니다. Flow cytometry와 IHC(P)에서 검증되었으며, 고순도의 액상 형태로 제공됩니다. 4°C 보관, 동결 금지.
- 카탈로그번호
- 606-0452-82
- 판매단위
- pk
카탈로그
1개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific CD45R (B220) Monoclonal Antibody (RA3-6B2), Alexa Fluor 660, eBioscience
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100 |
| Flow Cytometry (Flow) | 0.5 µg/test |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rat / IgG2a, kappa |
| Recommended Isotype Control | Rat IgG2a kappa Isotype Control (eBR2a), Alexa Fluor™ 660, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | RA3-6B2 |
| Conjugate | Alexa Fluor™ 660 |
| Excitation / Emission Max | 663 / 691 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2896261 |
Available Formats
Unconjugated, Alexa Fluor 488, Alexa Fluor 532, Alexa Fluor 561, Alexa Fluor 700, APC, APC-eFluor 780, Biotin, Brilliant UV series, Brilliant Violet series, eFluor series, FITC, Functional Grade, NovaFluor series, PE, PE-Cyanine series, PerCP series, Super Bright series, and custom conjugation available.
Product Specific Information
The RA3-6B2 monoclonal antibody recognizes the exon A-restricted isoform of mouse CD45, a 220 kDa surface molecule. CD45R/B220 epitope is mainly expressed by B cell lineage from early Pro-B to mature B cells. Some activated T cells, lymphokine-activated killer (LAK) cells, NK cell progenitors, and certain mutant T cells also express this antigen.
- Applications Reported: Flow cytometric analysis
- Applications Tested: Tested by flow cytometric analysis of mouse splenocytes at ≤0.5 µg/test
A test is defined as the amount of antibody that stains a cell sample in 100 µL final volume. Optimal titration is recommended for specific assays.
Alexa Fluor 660 emits at 690 nm and is suitable for use on spectral cytometers, multiplexed with APC, Alexa Fluor 647, and Alexa Fluor 700.
Excitation: 662 nm Emission: 690 nm Laser: Red Laser
Target Information
CD45R (PTPRC) is a member of the protein tyrosine phosphatase (PTP) family involved in regulating cell growth, differentiation, mitotic cycle, and oncogenic transformation. It is expressed in hematopoietic cells and is essential for T- and B-cell receptor signaling. CD45 glycoprotein is crucial for lymphocyte development and antigen signaling, regulating Src-family kinases. Multiple isoforms exist due to alternative splicing, differing in extracellular domains while sharing identical transmembrane and cytoplasmic domains. CD45 also modulates integrin-mediated signaling and apoptosis. Dysfunction is associated with immunodeficiency and hepatitis C.
For Research Use Only. Not for use in diagnostic procedures.
제품 이미지
Thermo Fisher Scientific 상품 둘러보기
전체보기
문의
0개 · 배송·재고 문의는 실시간 상담이 빠릅니다아직 등록된 문의가 없어요.