Thermo Fisher Scientific CD45 Monoclonal Antibody (HI30), Alexa Fluor 660, eBioscience
인간 CD45 모든 아이소폼을 인식하는 HI30 단클론 항체로, Alexa Fluor 660 형광 표지되어 있습니다. Flow Cytometry 분석에 최적화되어 있으며, 5 µL/test로 사용됩니다. 적혈구 및 혈소판을 제외한 모든 조혈세포 표지에 적합합니다.
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Thermo Fisher Scientific CD45 Monoclonal Antibody (HI30), Alexa Fluor 660, eBioscience
Applications and Tested Dilution
- Application: Flow Cytometry
- Tested Dilution: 5 µL (0.25 µg)/test
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Recommended Isotype Control | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), Alexa Fluor 660, eBioscience |
| Class | Monoclonal |
| Type | Antibody |
| Clone | HI30 |
| Conjugate | Alexa Fluor 660 |
| Excitation / Emission Max | 663 / 691 nm |
| Form | Liquid |
| Concentration | 5 µL/test |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2896262 |
Additional Formats Available
- Unconjugated
- Alexa Fluor 532
- Alexa Fluor 561
- Alexa Fluor 647
- APC
- APC-eFluor 780
- Biotin
- Brilliant UV 395 / 496 / 563 / 615 / 661 / 737 / 805
- Brilliant Violet 421 / 480 / 605 / 650 / 711
- eFluor 450 / 506
- FITC
- NovaFluor Blue 510 / 585 / 690
- NovaFluor Red 710 / 725 / 755
- NovaFluor Yellow 590 / 660 / 730 / 755
- PE / PE-Cyanine5 / PE-Cyanine5.5 / PE-Cyanine7 / PE-eFluor 610
- PerCP-Cyanine5.5 / PerCP-eFluor 710
- Request custom conjugation available
Product Specific Information
The HI30 monoclonal antibody reacts with all isoforms of human CD45 (Leukocyte Common Antigen, LCA).
CD45 is expressed by all hematopoietic cells excluding circulating erythrocytes and platelets.
The cytoplasmic portion of CD45 possesses tyrosine phosphatase activity, playing an essential role in lymphocyte activation.
- Applications Reported: Flow cytometric analysis
- Applications Tested: Pre-diluted and validated for flow cytometry of human peripheral blood cells
- Usage: 5 µL (0.25 µg) per test (100 µL final volume, 10⁵–10⁸ cells/test)
- Fluorophore: Alexa Fluor 660 (Emits at 690 nm)
- Laser: Red Laser (Excitation 662 nm, Emission 690 nm)
- Multiplexing: Compatible with APC, Alexa Fluor 647, Alexa Fluor 700
Target Information
CD45 (LCA) is a receptor-type protein tyrosine phosphatase expressed on all nucleated hematopoietic cells, comprising ~10% of lymphocyte surface proteins.
Absent on non-hematopoietic tissues, CD45 regulates Src-family kinases and antigen receptor signaling, critical for T- and B-cell activation.
Multiple isoforms arise via alternative splicing, differing in extracellular domains but sharing transmembrane and cytoplasmic regions.
CD45 modulates integrin-mediated signaling, apoptosis, and immune response regulation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without authorization.
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