Thermo Fisher Scientific CD11b Monoclonal Antibody (ICRF 44)

Applications

Tested Dilution

Publications

Immunohistochemistry (Frozen) (IHC (F))

1:100-1:1,000

-

Flow Cytometry (Flow)

1:10-1:100

View 2 publications 2 publications

Immunoprecipitation (IP)

Assay-dependent

-

Neutralization (Neu)

-

View 1 publication 1 publication

Product Specifications

Species Reactivity

Baboon, Cynomolgus monkey, Human, Non-human primate, Rhesus monkey

Published species

Human, Pig

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

ICRF 44

Immunogen

Rheumatoid synovial cells and human monocytes. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:CD11b,uniProtId:P11215-1,ncbiNodeId:9606,antigenRange:1-1152,antigenLength:1152,antigenImageFileName:MA1-90974_CD11b_P11215-1_House_mouse.svg,antigenImageFileNamePDP:MA1-90974_CD11b_P11215-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

PBS

Contains

0.09% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_1957540

Product Specific Information

MA1-90974 detects Integrin alpha M from non-human primate, human samples.

Target Information

CD11b (integrin alpha-M, ITGAM, integrin alpha-X, ITGAX) is a 165 kDa adhesion molecule that associates non-covalently with integrin beta-2 (CD18). The CD11b/CD18 heterodimeric complex is also known as integrin alpha-M beta-2, Mac-1, and CR3 (complement receptor 3). CD11b is expressed on the surface of monocytes/macrophages, granulocytes, activated lymphocytes, a subset of NK cells, a subset of dendritic cells, and microglia in the brain. CD11b/CD18 functions as the receptor for ICAM-1 (CD54), ICAM-2 (CD102), ICAM-4 (CD242), CD14, CD50, CD23, heparin, iC3b, fibrinogen, and Factor X -these adhesions are critical for cell-cell and cell-matrix interactions. CD11b is expressed on 8% of spleen cells, 44% of bone marrow cells, and less than 1% of thymocytes, and is commonly used as a microglial marker in nervous tissue. The expression of CD11b increases during monocyte maturation and expression levels vary on tissue macrophages. Further, peritoneal macrophages are reported to express higher levels of CD11b than splenic macrophages. Diseases associated with CD11b dysfunction include systemic lupus erythematosus 6 and ITGAM-related susceptibility to systemic lupus erythematosus.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.