Thermo Fisher Scientific Calreticulin Monoclonal Antibody (FMC 75)

Applications

Tested Dilution

Publications

Western Blot (WB)

0.5 µg/mL

-

Immunohistochemistry (IHC)

2 µg/mL

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

Assay-dependent

-

Flow Cytometry (Flow)

1:100

-

Product Specifications

Species Reactivity

Hamster, Human, Non-human primate

Published species

Mouse

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

FMC 75

Immunogen

Calreticulin-maltose binding fusion protein

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

PBS, pH 7.2, with 0.1mM PMSF, 50% glycerol

Contains

no preservative

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_1954979

Product Specific Information

Recommended positive controls: HeLa heat shocked cell lysate.

Target Information

Calr or Calreticulin is a calcium-binding chaperone that promotes folding, oligomeric assembly and quality control in the endoplasmic reticulum (ER) via the calreticulin/calnexin cycle. Calr, a lectin, interacts transiently with almost all of the monoglucosylated glycoproteins that are synthesized in the ER. Calr also interacts with with the DNA-binding domain of NR3C1 and mediates its nuclear export. It may also be involved in maternal gene expression regulation and oocyte maturation via the regulation of calcium homeostasis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.