Thermo Fisher Scientific ERK2 Monoclonal Antibody (6F8)

Applications

Tested Dilution

Publications

Western Blot (WB)

1-3 µg/mL

-

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:500

-

Immunocytochemistry (ICC/IF)

1:200

-

Flow Cytometry (Flow)

1-10 µg/mL

-

Immunoprecipitation (IP)

2 µg/500 µL

-

ChIP assay (ChIP)

1-3 µL

-

Product Specifications

Species Reactivity

Dog, Human, Mouse, Non-human primate, Rat

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

6F8

Immunogen

Protein expressed in 293T cells transfected with human MAPK1 expression vector

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS with 1mg/mL BSA, 30% glycerol

Contains

0.05% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2536743

Product Specific Information

MA1-099 has been successfully used in Western blot, ChIP, flow cytometry, immunofluorescence, immunohistochemistry and immunoprecipitation applications, and reacts with human, dog, rat, mouse and monkey samples.

MA1-099 has successfully been used in western blot to detect p42 ERK in HeLa, NRK and MDCK cell lysates.

As demonstrated by a Western blot using recombinant ERK1 and ERK2 proteins, MA1-099 can also detect ERK1, but detects ERK2 at much lower concentrations. As demonstrated by a Western blot using a panel of lysates from various species, MA1-099 only detects endogenous ERK2.

Target Information

MAPK1 (ERK2) is a serine/threonine kinase that acts as an important activator of p90 RSK, MSK, ELK1, and Stat3, and is involved in the MAPK pathway. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. The activation of this kinase requires its phosphorylation by upstream kinases. Upon activation, this kinase translocates to the nucleus of the stimulated cells, where it phosphorylates nuclear targets.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.