Thermo Fisher Scientific NSP1 Monoclonal Antibody (32D6)
Thermo Fisher Scientific의 NSP1 Monoclonal Antibody (32D6)는 효모 Nsp1p 단백질을 특이적으로 검출하는 항체로, WB, IHC, ICC/IF, IP 등 다양한 분석에 적합합니다. 고특이성 및 재현성 높은 결과를 제공하며, 연구용으로 최적화된 제품입니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:10,000–1:50,000 |
| Immunohistochemistry (IHC) | 1:1,000–1:10,000 |
| Immunocytochemistry (ICC/IF) | 1:1,000–1:10,000 |
| Immunoprecipitation (IP) | Assay-dependent |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Yeast |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 32D6 |
| Immunogen | Yeast nuclear preparation |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | Not determined |
| Storage Buffer | Tissue culture supernatant |
| Contains | 5 mM sodium azide |
| Storage Conditions | Store at 4°C short term; for long term, store at -20°C avoiding freeze/thaw cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_1077314 |
Product Specific Information
MA1-10029 detects Nsp1p from S. cerevisiae samples. To confirm high affinity for Nsp1p, strain RS453 (shortened isoform) was compared to wildtype strain BJ5465.
NSP1 in RS453 lacks six FXFG repeats, encoding a protein migrating at ~85 kDa on SDS-PAGE gels. Wildtype Nsp1p has a predicted MW of 86.5 kDa, typically migrating at ~100–108 kDa. SDS-PAGE results confirmed specificity for Nsp1p: wildtype at ~108 kDa and RS453 at ~91 kDa.
- Western Blot (WB): Use MA1-10029 diluted 1:10,000 (cell lysates) to 1:50,000 (nuclear fractions) with ECL detection.
- Other WB methods: Use 1:1,000–1:5,000.
- Immunofluorescence (IF): Use 1:1,000–1:10,000 on yeast cells.
Target Information
Nucleoporin NSP1 is a component of the yeast nuclear pore complex (NPC). NPC components (nucleoporins, NUPs) serve as structural elements and docking sites for nuclear transport factors.
Directional transport is facilitated by FG-repeat affinity gradients and GSP1/GSP2 GTPase gradients across the nuclear envelope.
NSP1 is essential for multiple nuclear transport pathways, including poly(A)+ RNA, tRNA, pre-ribosome, SRP, and protein transport.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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