Thermo Fisher Scientific NOP1 Monoclonal Antibody (28F2)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1:2,000-1:20,000

-

Immunoprecipitation (IP)

2-4 µL/lysate

-

Product Specifications

Species Reactivity

Yeast

Published species

Yeast

Host/Isotype

Mouse / IgG3

Class

Monoclonal

Type

Antibody

Clone

28F2

Immunogen

Yeast nuclear preparation. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:NOP1,uniProtId:P15646-1,ncbiNodeId:-23,antigenRange:1-327,antigenLength:327,antigenImageFileName:MA1-10025_NOP1_P15646-1_House_mouse.svg,antigenImageFileNamePDP:MA1-10025_NOP1_P15646-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

Conc. Not Determined

Storage buffer

tissue culture supernatant

Contains

5mM sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_1077286

Product Specific Information

MA1-10025 detects Yeast NOP-1 from S. cerevisiae samples and does not cross-react with fibrillarin in other species.

For chemiluminescent Western blot using yeast samples, use a dilution of 1:10,000 for cell lysates and 1:25,000 for nuclear fractions. For non-ECL detection methods, use a dilution of 1:1000-1:5000.

Target Information

Nop1p is the yeast homologue of human fibrillarin, a component of the nucleolar small nuclear ribonucleoprotein (snRNP) particle.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.