Thermo Fisher Scientific PIGY Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

5 µg/mL

-

Immunohistochemistry (IHC)

2.5 µg/mL

-

Product Specifications

Species Reactivity

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A 16 amino acid peptide near the carboxy terminus of human PIG-Y. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:PIGY,uniProtId:Q3MUY2-1,ncbiNodeId:9606,antigenRange:71,antigenLength:71,antigenImageFileName:PA5-20651_PIGY_Q3MUY2-1_Rabbit.svg,antigenImageFileNamePDP:PA5-20651_PIGY_Q3MUY2-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS

Contains

0.02% sodium azide

Storage conditions

Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_11153820

Product Specific Information

Despite its predicted molecular weight, PIG-Y often migrates at 28-30kDa in SDS-PAGE. A suggested positive control is A-20 cell lysate.

PA5-20651 can be used with blocking peptide PEP-0771.

Target Information

Glycosylphosphatidylinositol (GPI) lipid anchoring is an important post-translational modification of proteins that takes place in the endoplasmic reticulum. The synthesis of GPI is initiated by GPI-N-acetylglucosaminyltransferase (GPI-GnT), a complex of proteins including PIG-A, PIG-H, PIG-C, GPI1, and DPM2. PIG-Y, the mammalian homolog to yeast Eri1p, is also thought to be involved in the biosynthesis of GPI. The PIG-Y gene encodes two proteins, one of which arises from leaky scanning of the mRNA.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.