Thermo Fisher Scientific PIGY Polyclonal Antibody
PIGY 단백질을 인식하는 Rabbit Polyclonal 항체로, WB와 IHC에 적합합니다. Human 및 Mouse 시료에 반응하며, 항원 친화 크로마토그래피로 정제되었습니다. 액상 형태로 제공되며, 2–8°C 보관 가능합니다. 연구용으로만 사용됩니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 5 µg/mL |
| Immunohistochemistry (IHC) | 2.5 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A 16 amino acid peptide near the carboxy terminus of human PIG-Y |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage buffer | PBS |
| Contains | 0.02% sodium azide |
| Storage conditions | Maintain refrigerated at 2–8°C for up to 3 months. For long term storage store at -20°C |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_11153820 |
Product Specific Information
Despite its predicted molecular weight, PIG-Y often migrates at 28–30 kDa in SDS-PAGE.
A suggested positive control is A-20 cell lysate.
PA5-20651 can be used with blocking peptide PEP-0771.
Target Information
Glycosylphosphatidylinositol (GPI) lipid anchoring is an important post-translational modification of proteins that takes place in the endoplasmic reticulum. The synthesis of GPI is initiated by GPI-N-acetylglucosaminyltransferase (GPI-GnT), a complex of proteins including PIG-A, PIG-H, PIG-C, GPI1, and DPM2. PIG-Y, the mammalian homolog to yeast Eri1p, is also thought to be involved in the biosynthesis of GPI. The PIG-Y gene encodes two proteins, one of which arises from leaky scanning of the mRNA.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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