Thermo Fisher Scientific Bcl-B Polyclonal Antibody
Human Bcl-B 단백질을 인식하는 Rabbit Polyclonal Antibody로, WB, IHC, ICC/IF에 사용 가능. 항원 친화 크로마토그래피로 정제되었으며, 비결합형 액상 형태. 세포 사멸 연구 및 BCL-2 패밀리 단백질 분석에 적합.
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Thermo Fisher Scientific Bcl-B Polyclonal Antibody
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:3,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:100–1:1,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fragment corresponding to a region within amino acids 1–194 of Human Bcl-B |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.51 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | 0.1M Tris glycine, pH 7, with 20% glycerol |
| Contains | 0.01% thimerosal |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2547341 |
Product Specific Information
- Recommended positive controls: A431, H1299, HeLa, HepG2
- Store product as a concentrated solution.
- Centrifuge briefly prior to opening the vial.
Target Information
The protein encoded by this gene belongs to the BCL-2 protein family. BCL-2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators involved in various cellular activities.
This protein contains conserved BH4, BH1, and BH2 domains and interacts with other BCL-2 family members including BCL2, BCL2L1/BCL-X(L), and BAX.
Overexpression suppresses cell apoptosis by preventing cytochrome C release from mitochondria, thereby inhibiting caspase-3 activation.
The mouse homolog interacts with Apaf1 and forms complexes with Caspase 9, suggesting involvement in the APAF1 and CASPASE 9 apoptotic pathways.
Safety Information
⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm.
For more information, visit www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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