Thermo Fisher Scientific Human FGF-4, Animal-Free Recombinant Protein, PeproTech
인체 유래 FGF-4 재조합 단백질로, 동물 유래 성분이 없는 고순도 제품입니다. 세포 증식 및 분화 연구, 줄기세포 성장 및 조직 재생 연구에 적합합니다. E. coli 발현, ≥95% 순도, 19.7 kDa 분자량.
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Applications
Functional Assay (Functional)
- Assay-dependent
In vitro Assay (IV)
- Refer to publications (10 available)
Miscellaneous PubMed (Misc)
- Refer to publication (1 available)
Product Specifications
| 항목 | 내용 |
|---|---|
| Species | Human |
| Published Species | Human, Mouse, Pig |
| Expression System | E. coli |
| Amino Acid Sequence | GRGGAAAPTA PNGTLEAELE RRWESLVALS LARLPVAAQP KEAAVQSGAG DYLLGIKRLR RLYCNVGIGF HLQALPDGRI GGAHADTRDS LLELSPVERG VVSIFGVASR FFVAMSSKGK LYGSPFFTDE CTFKEILLPN NYNAYESYKY PGMFIALSKN GKTKKGNRVS PTMKVTHFLP RL |
| Molecular Weight | 19.7 kDa |
| Class | Recombinant |
| Type | Protein |
| Purity | ≥ 95% by SDS-PAGE gel and HPLC analyses |
| Endotoxin Concentration | < 0.1 EU/µg |
| Activity | Determined by a cell proliferation assay using Balb/c 3T3 cells. Expected ED50 ≤ 10 ng/ml, specific activity ≥ 1 × 10^5 units/mg |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Purification | Purified |
| Contains | No preservative |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient |
Product Specific Information
- Catalog No. AF-100-31-1MG is provided as 2 × 500 µg (AF-100-31-500UG).
- Recombinant Human FGF-4 is a 19.7 kDa protein consisting of 182 amino acid residues.
- Shipped at ambient temperature. For storage, handling, and reconstitution information, please refer to the lot-specific Certificate of Analysis.
Target Information
Fibroblast Growth Factor 4 (FGF4) is a member of the FGF family with broad mitogenic and cell survival activities. It plays key roles in stem cell growth and survival during embryogenesis, tissue regeneration, and carcinogenesis. FGF4 exhibits strong oncogenic transforming activity and acts as a potent angiogenic factor, expressed in several highly vascularized tumors and adult mouse testis, intestine, and brain.
Mouse studies suggest FGF4 functions in bone morphogenesis and limb development via the sonic hedgehog (SHH) signaling pathway. It also regulates neural progenitor cell proliferation and neuronal differentiation. Recent findings indicate a growth-promoting role in human embryonic stem cells and a feedback inhibition mechanism through a novel splice isoform that may promote differentiation at later developmental stages.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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