Thermo Fisher Scientific RhoA Recombinant Rabbit Monoclonal Antibody (SN0612)
RhoA 단백질을 인식하는 recombinant rabbit monoclonal antibody로, Western blot, ICC/IF, Flow cytometry에 적합합니다. 높은 특이성과 재현성을 제공하며, HEK293 발현 시스템에서 생산된 IgG 형식의 항체입니다. 연구용으로만 사용 가능합니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:2,000 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| Flow Cytometry (Flow) | 1:50–1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | SN0612 |
| Immunogen | Synthetic peptide within Human RhoA (aa 144–193) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | TBS, pH 7.4, with 40% Glycerol, 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2809548 |
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The antibody DNA sequences from immunoreactive rabbits are cloned and expressed in HEK293 cells. Individual clones are screened to select optimal candidates for production.
Recombinant rabbit monoclonal antibodies offer several advantages:
- Enhanced specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulation
- Broader immunoreactivity due to the rabbit immune repertoire
Target Information
RhoA regulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers. It plays a critical role in cytokinesis by contributing to myosin contractile ring formation and cleavage furrow development.
RhoA is essential for apical junction formation in keratinocyte adhesion and acts as a target for Yersinia pestis and Yersinia pseudotuberculosis toxins. It activates PKN2 kinase and may regulate PLCE1 activity.
RhoA is also involved in SPATA13-mediated cell migration and adhesion dynamics, and participates in the MEMO1-RHOA-DIAPH1 signaling pathway, which stabilizes microtubules at the cell cortex through ERBB2-dependent mechanisms.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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