Thermo Fisher Scientific iNOS Recombinant Rat Monoclonal Antibody (CXNFT), Alexa Fluor Plus 647
Alexa Fluor Plus 647로 표지된 iNOS 랫트 단클론항체(CXNFT). Western blot, ICC/IF, Flow cytometry에 적합. 고신호 대비와 일관된 로트 간 재현성 제공. 4°C에서 암소 보관, 동결 금지. 연구용 전용.
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- 740025TP647x (2개 옵션)
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Applications and Tested Dilutions
| Application | Tested Dilution / Amount |
|---|---|
| Western Blot (WB) | 1:200 |
| Immunocytochemistry (ICC/IF) | 1:500 |
| Flow Cytometry (Flow) | 0.25 µg/test |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Host / Isotype | Rat / IgG2a, kappa |
| Expression System | Expi293 |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | CXNFT |
| Conjugate | Alexa Fluor™ Plus 647 |
| Excitation / Emission Max | 658 / 675 nm |
| Form | Liquid |
| Concentration | 1.0 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | Proprietary buffer, pH 6.8 |
| Contains | 0.008% Bromonitrodioxane, 0.008% Methylisothiazolone |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Wet ice |
| Filtration | 0.2 µm post-manufacturing filtered |
Additional Formats
Product Specific Information
Alexa Fluor™ Plus recombinant antibodies use advanced dye chemistry for superior brightness and signal-to-noise ratio compared to conventional Alexa Fluor antibodies. These recombinant antibodies ensure improved specificity and consistent lot-to-lot performance by expressing cloned antibody genes in vitro.
Usage Note:
Before use, briefly centrifuge the conjugate solution and use only the supernatant to minimize nonspecific background caused by aggregates.
Applications Tested:
This CXNFT antibody has been validated for western blot, immunocytochemistry, and flow cytometry analysis of RAW 264.7 cells treated with LPS.
- ICC: 2 µg/mL
- Flow cytometry: ≤ 0.25 µg/test (100 µL volume, 10⁵–10⁸ cells/test recommended)
Titrate antibody concentration for optimal assay performance.
Excitation: 658 nm
Emission: 675 nm
Laser: Red Laser
Target Information
Inducible nitric oxide synthase (iNOS) produces nitric oxide (NO), a gaseous signaling molecule involved in vasorelaxation, neurotransmission, and cytotoxicity. iNOS expression is inducible in liver and other tissues by lipopolysaccharide and cytokines. NOS enzymes catalyze the oxidation of L-arginine to L-citrulline and NO, with three isoforms identified: nNOS (type I), iNOS (type II), and eNOS (type III).
iNOS is expressed in macrophages, hepatocytes, synoviocytes, and smooth muscle cells. Its activity increases in response to cytokines such as IFN-γ, TNF, IL-1, IL-2, and LPS. Dysregulation of iNOS is associated with conditions such as achalasia and impotence.
For Research Use Only. Not for use in diagnostic procedures or resale without authorization.
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