Thermo Fisher Scientific CD4 Recombinant Rat Monoclonal Antibody (4SM95)

Applications

Tested Dilution

Publications

Immunohistochemistry (Paraffin) (IHC (P))

1:200

-

Immunocytochemistry (ICC/IF)

1:800

-

Flow Cytometry (Flow)

0.5 µg/test

-

Product Specifications

Species Reactivity

Human, Mouse

Host/Isotype

Rat / IgG1, kappa

Expression System

Expi293

Class

Recombinant Monoclonal

Type

Antibody

Clone

4SM95

Conjugate

Unconjugated Unconjugated Unconjugated

View additional formats

• Alexa Fluor Plus 488
• Request custom conjugation

Form

Liquid

Concentration

1.0 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.4

Contains

0.09% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

Product Specific Information

Recombinant rat monoclonal antibodies are produced using in vitro expression systems. Recombinant antibodies are produced using specific genes that code for the desired antibodies. These genes are cloned into an expression vector and expressed in vitro. The advantages of recombinant antibodies include better specificity and lot-to-lot consistency.

It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Target Information

The CD4 antigen is involved in the recognition of MHC class II molecules and is a co-receptor for HIV. CD4 is primarily expressed in a subset of T-lymphocytes, also referred to as T helper cells, but may also be expressed by other cells in the immune system, such as monocytes, macrophages, and dendritic cells. At the tissue level, CD4 expression may be detected in thymus, lymph nodes, tonsils, and spleen, and also in specific regions of the brain, gut, and other non-lymphoid tissues. CD4 functions to initiate or augment the early phase of T-cell activation through its association with the T-cell receptor complex and protein tyrosine kinase, Lck. It may also function as an important mediator of direct neuronal damage in infectious and immune-mediated diseases of the central nervous system. Multiple alternatively spliced transcripts have been identified in this gene [RefSeq, July 2017].

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.