Zenogen Pharma STEM-CELLBANKER® GMP grade

Zenogen Pharma STEM-CELLBANKER® GMP grade

For inquiries including COA (Certificate of Analysis), please contact:
zenogen_pharma-post@zenoaq.jp

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Description

STEM-CELLBANKER® is a chemically defined freezing solution optimized for stem cells, iPS cells, and other valuable cells. It is serum-free and animal component-free, containing only USP, EP, JP graded ingredients or JP excipients.
Ready-to-use formulation ensures safe and efficient cryopreservation.
Manufactured under JP, EU, US, and PIC/S GMP guidelines.

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Advantages

• High cell viability while maintaining pluripotency and normal karyotype.
• Effective cryopreservation for tissues without DNA/RNA damage.
• Free from animal-derived contamination, ensuring batch stability.
• Simple and cost-effective process (direct freezing at -80°C, no programmable freezer needed).
• Produced under strict JP GMP compliance.

Regulatory Registration:
MF registered within PMDA (2013) and BBMF within CBER/FDA (2013).

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Package

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Application

① Cryopreservation analysis on hESC and iPS cells

By Department of Clinical Science, Intervention and Technology, Karolinska Institutet

Experimental Summary:
| Cell Lines | STEM-CELLBANKER | Conventional Medium |
|-------------|----------------|---------------------|
| HS293 | 93% | 49% |
| HS306 | 96% | 49% |
| ChiPSA | 90% | 50% |

STEM-CELLBANKER® showed significantly higher survival rates compared to conventional freezing medium (10% DMSO in serum replacement medium).
Cell pluripotency and normal karyotype were maintained.

Cell count post thawing (Passage comparison):

• HS293, HS306, ChiPSA showed consistent recovery across Passage 1–3.
• STEM-CELLBANKER® maintained higher cell counts post-thawing compared to controls.

Grateful acknowledgment to Prof. Outi Hovatta and Ms. Frida Holm.

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② Cryopreservation analysis on Mesenchymal Stem Cells (MSCs) and Endothelial Progenitor Cells (EPCs)

By Regenerative Medicine Faculty, University of Tsukuba

Cells were frozen directly at -80°C for 3 days, then transferred to -192°C for 72 days.
High viability and maintained differentiation ability confirmed.
References: MSCs, EPCs

Acknowledgment to Prof. Osamu Ohneda and Dr. Masumi Nagano.

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③ Cryopreservation analysis on Mouse ES cell (129SV)

By ZENOAQ Cell Engineering Team

Comparison of CELLBANKER® series:
CELLBANKER1, 1plus, 2, and STEM-CELLBANKER showed varying performance (scale 20–100).
STEM-CELLBANKER demonstrated superior viability.

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Protocol (CELLBANKER® Series)

Freezing Procedure

1. Ensure healthy, contamination-free cell culture.
2. Count viable cells.
3. Centrifuge (3–5 min, 1,000–2,000 rpm, 4°C).
4. Suspend cells in CELLBANKER medium (1 mL for 5×10⁵–5×10⁶ cells).
5. Dispense into labeled cryovials.
6. Freeze directly at -80°C.
7. Optionally transfer to liquid nitrogen after 24 hours.

Procedure for Use (Summary Table):

Thawing Procedure

1. Thaw quickly in 37°C water bath.
2. Dilute each 1 mL with 10 mL culture medium.
3. Centrifuge (3–5 min, 1,000–2,000 rpm, 4°C).
4. Resuspend and plate cells.
5. Continue standard culture.

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④ Cryopreservation of Mouse Kidney Tissue

By ZENOAQ Cell Engineering Team

Mouse kidney (5 mm squares) cryopreserved with 1 mL STEM-CELLBANKER at -80°C for one week.
Post-thawing, tissues fixed with 10% formalin and stained (H&E, Active Caspase 3).
Both fresh and cryopreserved samples showed negligible necrosis/apoptosis, confirming tissue integrity.

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⑤ Preservation of mRNAs after Cryopreservation

mRNA levels in mouse lung fibroblast cells remained within ±1.6-fold difference after cryopreservation at -80°C for one week.
93% of transcripts preserved, confirming minimal RNA degradation.

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References

• Shimazu et al. (2015) Cytotherapy 17:593–600
• Nakagawa M et al. (2014) Scientific Reports 4:359
• Miyamoto Y et al. (2012) Cell Medicine 3:89–95
• Saliem M et al. (2012) World J Hepatol 4(5):176–83
• Holm et al. (2010) Hum Reprod 25(5):1271–9
• Nakamura A et al. (2010) Med Mol Morphol 43:107–115

Protocol (human ES and iPS cells)

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Storage and Stability

Store at 2–8°C or below -20°C.
Stable for 3 years under unopened condition at 2–8°C.

SDS: STEM-CELLBANKER® GMP grade

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Disclaimer

STEM-CELLBANKER® GMP grade is not a pharmaceutical.
ZENOAQ and ZENOGEN PHARMA make no warranty as to fitness or merchantability unless intended for research use.

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제품 이미지

1. Main Product Image
   

OCR 텍스트:
100 mL Cryopreservation Solution for Cells and Tissues
STEM-CELLBANKER GMP grade
NIPPON ZENYAKU KOGYO CO., LTD.
1-1 Tairanoue Sasagawa, Asaka-machi, Koriyama, Fukushima 963-0196 Japan
(또한 소병 라벨 동일 내용, 측면 표기 “210208 EXP.10”)

2. Cryopreservation Analysis (hESC/iPS)
   

3. Cell Count Post Thawing Graph
   

4. Mouse ES Cell Comparison
   

5. Protocol Diagram
   

6. Mouse Kidney Tissue Cryopreservation
   
   
   
   

7. mRNA Preservation Graph