Thermo Fisher Scientific Tau Monoclonal Antibody (TAU-5)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500

View 79 publications 79 publications

Immunohistochemistry (IHC)

-

View 11 publications 11 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

-

Immunohistochemistry (Frozen) (IHC (F))

1:500

View 2 publications 2 publications

Immunocytochemistry (ICC/IF)

Assay-dependent

View 5 publications 5 publications

ELISA (ELISA)

-

View 2 publications 2 publications

Immunoprecipitation (IP)

Assay-dependent

View 5 publications 5 publications

in situ PLA (PLA)

-

View 1 publication 1 publication

Dot blot (DB)

-

View 1 publication 1 publication

Immunoelectrophoresis (IE)

-

View 1 publication 1 publication

Miscellaneous PubMed (Misc)

-

View 5 publications 5 publications

Product Specifications

Species Reactivity

Bovine, Human, Mouse, Sheep, Rat

Published species

Bovine, Human, Mouse, Non-human primate, Rat

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

TAU-5

Immunogen

Purified bovine microtubule-associated proteins. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Tau,uniProtId:P29172-1,ncbiNodeId:9913,antigenRange:1-448,antigenLength:448,antigenImageFileName:AHB0042_Tau_P29172-1_House_mouse.svg,antigenImageFileNamePDP:AHB0042_Tau_P29172-1_House_mouse_PDP.jpeg,sortOrder:4}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

purified

Storage buffer

PBS, pH 7.4

Contains

15mM sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2536235

Target Information

Tau is a neuronal microtubule-associated protein found predominantly on axons. The function of Tau is to promote tubulin polymerization and stabilize microtubules. The C-terminus binds axonal microtubules while the N- terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both. Axonal polarity is predetermined by TAU/MAPT localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton while the longer isoforms may preferentially play a role in its stabilization. In its hyper-phosphorylated form, Tau is the major component of paired helical filaments (PHF), the building block of neurofibrillary lesions in Alzheimers diseases (AD) brain. Hyper-phosphorylation impairs the microtubule binding function of Tau, resulting in the destabilization of microtubules in AD brains, ultimately leading to the degeneration of the affected neurons. Numerous serine/threonine kinases phosphorylate Tau, including GSK-3beta, protein kinase A (PKA), cyclin-dependent kinase 5 (cdk5) and casein kinase II. Hyper-phosphorylated Tau is found in neurofibrillary lesions in a range and other central nervous system disorders such as Picks disease, frontotemporal dementia, cortico-basal degeneration and progressive supranuclear palsy.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.