Thermo Fisher Scientific LIMK1 Monoclonal Antibody (OTI6B4), TrueMAB
LIMK1 단백질을 인식하는 단일클론 항체로, Western blot, IHC, ICC/IF, Flow Cytometry 등 다양한 응용에 적합. Mouse IgG2a 아이소타입, 동결건조 형태이며 높은 특이성과 재현성을 제공. 연구용으로만 사용 가능.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:150 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| Flow Cytometry (Flow) | 1:100 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Dog, Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI6B4 |
| Immunogen | Full-length human recombinant protein of human LIMK1 produced in HEK293T cell |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments using this carrier-free antibody, perform an additional desalting step (recommended: Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
LIMK1 is a serine/threonine kinase that regulates actin cytoskeletal dynamics via phosphorylation of cofilin. It is also implicated in brain development. LIM domains are conserved cysteine-rich structures containing two zinc fingers, which likely mediate protein–protein interactions.
LIM kinase-1 and LIM kinase-2 form a subfamily characterized by two N-terminal LIM motifs and a C-terminal kinase domain. LIMK1 may function in intracellular signaling pathways and is associated with cognitive deficits observed in Williams syndrome due to LIMK1 hemizygosity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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