Thermo Fisher Scientific Cathepsin O Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:3,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1,000

-

Immunocytochemistry (ICC/IF)

1:100-1:1,000

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fragment corresponding to a region within amino acids 41 and 279 of Human Cathepsin O if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Cathepsin O,uniProtId:P43234-1,ncbiNodeId:9606,antigenRange:41-279,antigenLength:321,antigenImageFileName:PA5-28285_Cathepsin_O_P43234-1_Rabbit.svg,antigenImageFileNamePDP:PA5-28285_Cathepsin_O_P43234-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

0.1M tris glycine, pH 7, with 10% glycerol

Contains

0.01% thimerosal

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2545761

Product Specific Information

Recommended positive controls: HepG2, HCT116.

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

The cathepsin family of proteolytic enzymes contains several diverse classes of proteases. The cysteine protease class comprises cathepsins B, L, H, K, S, and O. The aspartyl protease class is composed of cathepsins D and E. Cathepsin G is in the serine protease class. Most cathepsins are lysosomal and each is involved in cellular metabolism, participating in various events such as peptide biosynthesis and protein degradation. Cathepsin O maintains the structural features characteristic of other cysteine proteinases including the active site cysteine residue that is involved in covalent intermediate formation during peptide hydrolysis. Cathepsin O is an endoproteinase that may be involved in extracellular matrix degradation. Cathepsin O is abundantly expressed in the ovary, kidney and placenta with lower levels found in thymus and skeletal muscle.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.